PMID- 1548078
OWN - NLM
STAT- MEDLINE
DCOM- 19920423
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 60
IP  - 4
DP  - 1992 Apr
TI  - Identification of Candida albicans antigens reactive with immunoglobulin E 
      antibody of human sera.
PG  - 1550-7
AB  - Candida albicans antigens which reacted with immunoglobulin E (IgE) antibodies of 
      57 allergic patients were detected by immunoblotting. Of the various antigens, 
      the 175-, 125-, 46-, 43-, and 37-kDa antigenic components reacted most frequently 
      with the patient sera. To purify the major antigens, C. albicans cells were 
      fractionated. The 46-, 43-, and 37-kDa antigens were recovered in cytoplasmic 
      fractions, but the 175- and 125-kDa antigens were not recovered in any fraction. 
      The 46-, 43-, and 37-kDa antigens were purified from cytoplasmic fractions by 
      DEAE and P11 ion-exchange chromatography. Antigens were isolated by cutting bands 
      out of sodium dodecyl sulfate-polyacrylamide gels. The purified components 
      confirmed by immunoblotting were next processed for amino acid sequencing. Parts 
      of the sequences of the 46-, 43-, and 37-kDa antigens had significant levels of 
      homology with Saccharomyces cerevisiae glycolytic enzyme enolase, 
      phosphoglycerate kinase, and aldolase, respectively. Rabbit IgG antibodies 
      prepared against the 46- and 43-kDa antigens strongly cross-reacted with the 
      homologous proteins of S. cerevisiae. However, S. cerevisiae enolase and 
      phosphoglycerate kinase did not cross-react with IgE of patient sera. This result 
      suggests that IgE antibodies against only small parts of their epitopes are 
      elevated in the allergic patients. Since enolase is reported to be a major 
      antigen for systemic candidiasis, this enzyme may be the immunodominant protein 
      in both allergies and fungal infections.
FAU - Ishiguro, A
AU  - Ishiguro A
AD  - Laboratory of Medical Mycology, Nagoya University School of Medicine, Japan.
FAU - Homma, M
AU  - Homma M
FAU - Torii, S
AU  - Torii S
FAU - Tanaka, K
AU  - Tanaka K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, Fungal)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - OS382OHJ8P (Cyanogen Bromide)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Antigens, Fungal/*analysis/genetics/immunology/isolation & purification
MH  - Asthma/immunology
MH  - Blotting, Western
MH  - Candida albicans/*immunology
MH  - Cell Fractionation
MH  - Child
MH  - Child, Preschool
MH  - Chromatography, DEAE-Cellulose
MH  - Chromatography, Ion Exchange
MH  - Cyanogen Bromide
MH  - Dermatitis, Atopic/immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Radioallergosorbent Test
MH  - Sequence Homology, Nucleic Acid
PMC - PMC257030
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
PMCR- 1992/04/01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
PHST- 1992/04/01 00:00 [pmc-release]
AID - 10.1128/iai.60.4.1550-1557.1992 [doi]
PST - ppublish
SO  - Infect Immun. 1992 Apr;60(4):1550-7. doi: 10.1128/iai.60.4.1550-1557.1992.