PMID- 15481442
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20191026
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 80
IP  - 2
DP  - 2004 Aug
TI  - Simultaneous demonstration of clonal chromosome abnormalities and apoptosis in 
      individual marrow cells in myelodysplastic syndrome.
PG  - 140-5
AB  - Bone marrow samples from 18 patients with myelodysplastic syndrome (MDS) with 
      clonal cytogenetic abnormalities were characterized by combining fluorescence in 
      situ hybridization (FISH) with in situ end-labeling (ISEL) or with annexin V 
      staining and flow cytometry (AV/FLOW) to determine the clonal nature of 
      hematopoietic cells undergoing apoptosis in marrow cells. Apoptosis occurred in 
      both normal and clonal cells. However, the proportion of clonal cells identified 
      by FISH among apoptotic cells was lower than the proportion among nonclonal cells 
      in 17 of 18 patients, regardless of whether ISEL or AV/ FLOW was used to identify 
      apoptosis. This technique allows us to identify simultaneously clonality (as 
      determined by FISH) and apoptosis in individual cells and shows that although 
      apoptosis occurs predominantly in residual normal (FISH-negative) cells, a 
      proportion of clonal precursors in MDS marrow also die from programmed cell 
      death. Such a mechanism may be responsible for the generally slow expansion of 
      the clone in MDS.
FAU - Li, Xiao
AU  - Li X
AD  - Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, 
      Washington 98109-1024, USA.
FAU - Bryant, C Eileen
AU  - Bryant CE
FAU - Deeg, H Joachim
AU  - Deeg HJ
LA  - eng
GR  - CA87948/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
SB  - IM
MH  - Aged
MH  - *Apoptosis
MH  - Bone Marrow Cells/cytology/*pathology
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/pathology
EDAT- 2004/10/16 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/16 09:00
PHST- 2004/10/16 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/16 09:00 [entrez]
AID - 10.1532/ijh97.na0402 [doi]
PST - ppublish
SO  - Int J Hematol. 2004 Aug;80(2):140-5. doi: 10.1532/ijh97.na0402.