PMID- 15482515
OWN - NLM
STAT- MEDLINE
DCOM- 20050201
LR  - 20081121
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 15
IP  - 5
DP  - 2004 Oct
TI  - BCG vaccine modulates intestinal and systemic response to beta-lactoglobulin.
PG  - 408-14
AB  - Beta-Lactoglobulin (BLG) is a clinically important antigen in cow's milk and one 
      of the major allergens causing cow's milk allergy. Bacillus Calmette-Guerin (BCG) 
      vaccination has been suggested to modify immune response possibly decreasing the 
      risk of allergy to some antigens in both human and experimental animals. In the 
      present study, we have analyzed whether the early BCG vaccination has any effect 
      on the markers of systemic and gastrointestinal (GI) sensitization to BLG. We 
      immunized two groups of Hooded-Lister rat puppets with intraperitoneal injections 
      of native BLG at 43 and 62 days with pertussis vaccine as adjuvant, one group 
      receiving additionally BCG. The animals were then fed native and denatured milk 
      products twice weekly from 73 to 131 days of age, when they were killed. Control 
      group was not vaccinated and received normal rat forage. Total immunoglobulin E 
      (IgE) levels and BLG-specific IgG(1) and IgG(2a) concentrations were determined 
      in serum samples. Spontaneous interleukin (IL)-4 and interferon (IFN)-gamma 
      production from duodenal specimens were measured, and the inflammatory cells were 
      quantitated in specimens from different sections of the GI tract. Administration 
      of BCG simultaneously with BLG resulted in reduced IgE concentration in serum, 
      while the specific IgG(1) and IgG(2a) antibody responses and the spontaneous 
      secretion of IL-4 and IFN-gamma were not affected. Furthermore, BCG-induced 
      eosinophilic infiltration and increase of intraepithelial lymphocytes (IEL) in 
      the GI mucosa, and a trend toward increased number of lamina propria mononuclear 
      inflammatory cells in the colon (BCG compared with BLG, p = 0.09; BCG compared 
      with controls, p = 0.02). Controls showed increment of IgG(1) response in 
      comparison with the BLG group (p = 0.04) and increase of mucosal eosinophilic 
      infiltration. The BCG modified the response to BLG both at the systemic level as 
      shown by decrease of total IgE and at GI mucosa where increase of eosinophilic 
      infiltration and increased number of IEL were seen. Increment of IgG(1) level and 
      eosinophils in the controls might be related with the lack of modulatory effect 
      of pertussis vaccination. A shift of response toward the lower GI tract after BCG 
      immunization as shown by a trend for increase of mononuclear inflammatory cells 
      in colon lamina propria mimics disease development in some cases of clinical food 
      allergy, and emphasizes the need for evaluation of the changes in the whole GI 
      tract in food allergy models.
FAU - Rytkonen, Jani
AU  - Rytkonen J
AD  - Department of Pediatrics, University of Oulu, Oulu, Finland.
FAU - Karttunen, Tuomo J
AU  - Karttunen TJ
FAU - Karttunen, Riitta
AU  - Karttunen R
FAU - Valkonen, Kaija H
AU  - Valkonen KH
FAU - Bjorksten, Bengt
AU  - Bjorksten B
FAU - Kokkonen, Jorma
AU  - Kokkonen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (BCG Vaccine)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lactoglobulins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - BCG Vaccine/*immunology
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Interferon-gamma/blood
MH  - Interleukin-4/blood
MH  - Intestinal Mucosa/*immunology
MH  - Lactoglobulins/*immunology
MH  - Milk Hypersensitivity/immunology
MH  - Rats
EDAT- 2004/10/16 09:00
MHDA- 2005/02/03 09:00
CRDT- 2004/10/16 09:00
PHST- 2004/10/16 09:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/10/16 09:00 [entrez]
AID - PAI177 [pii]
AID - 10.1111/j.1399-3038.2004.00177.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2004 Oct;15(5):408-14. doi: 
      10.1111/j.1399-3038.2004.00177.x.