PMID- 15482672
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20181130
IS  - 0578-1310 (Print)
IS  - 0578-1310 (Linking)
VI  - 42
IP  - 9
DP  - 2004 Sep
TI  - [Effect of chemical modification of grafts on the survival improvement post 
      haploidentical bone marrow transplantation in mice].
PG  - 684-7
AB  - OBJECTIVE: Human leukocyte antigen (HLA) haploidentical bone marrow is a 
      potential source of donor to children for its availability. The drawback is 
      deleterious graft versus host disease (GVHD) reaction post transplantation 
      because of the incompatibility of HLA antigen expression between donors and 
      recipients, in which donor T lymphocyte is stimulated to proliferate and 
      differentiate. The methoxy polyethylene glycol (mPEG) is a kind of amphoteric 
      compound without immunogenicity, which was used to modify various proteins 
      covalently and to prepare the versatile blood type. If mPEG modification blocks 
      the activation of T cells in grafts, GVHD reaction probably would become less 
      serious and transplantation might become successful. The aim of this study was to 
      verify the improvement of haploidentical bone marrow transplantation (BMT) in a 
      murine model by using mPEG of certain concentration to modify the grafts. 
      METHODS: Male BALB/c mice were chosen as the donor, and female CB(6)F(1) mice as 
      the recipient. There were three groups of mPEG modification, non-modification and 
      irradiation control, and 20 mice in each group. The modified and non-modified 
      mixture of bone marrow and spleen cells (as T lymphocytes) were transplanted to 
      haploidentical lethally irradiated CB(6)F(1) mice via the tail vein. After the 
      transplant, the hematopoietic recovery, survival rate, acute graft versus host 
      disease (aGVHD) and chromosomal karyotype were analyzed and compared with 
      controls. RESULTS: Seventy-five percent (15/20) of mice survived in the group of 
      mPEG modification, while only 40% (8/20) survived in the group without the 
      modification (chi(2) = 5.01, P = 0.025). And 100% mice died in the group of the 
      irradiation control within 2 weeks. The hematopoietic recovery in the group of 
      mPEG modification was show n to be faster than that in the group without 
      modification (P < 0.05). Histopathological examination of the skin, liver and 
      intestine showed typical signs of aGVHD, but the GVHD grading in the group of 
      modification was less severe. The recipient mice in both groups of 
      transplantation surviving for more than 75 days showed complete donor-type 
      implantation by the chimerism examination. CONCLUSION: The modification of grafts 
      by mPEG could alleviate aGVHD and improve the survival rate of mice after the 
      haploidentical bone marrow transplantation.
FAU - Yang, Guang
AU  - Yang G
AD  - Department of Pediatrics, the General Hospital of PLA, Beijing 100853, China.
FAU - Tang, Suo-qin
AU  - Tang SQ
FAU - Zhang, Xiao-fei
AU  - Zhang XF
FAU - Liu, Li-zhen
AU  - Liu LZ
FAU - Huang, Dong-sheng
AU  - Huang DS
FAU - Wang, Jian-wen
AU  - Wang JW
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Ke Za Zhi
JT  - Zhonghua er ke za zhi = Chinese journal of pediatrics
JID - 0417427
RN  - 0 (HLA Antigens)
RN  - 0 (Immunosuppressive Agents)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9004-74-4 (monomethoxypolyethylene glycol)
SB  - IM
MH  - Animals
MH  - Bone Marrow/*drug effects/immunology
MH  - Bone Marrow Transplantation/*immunology
MH  - Disease Models, Animal
MH  - Graft Survival/*immunology
MH  - Graft vs Host Disease/immunology/prevention & control
MH  - HLA Antigens/immunology
MH  - Immunosuppressive Agents/*pharmacology/therapeutic use
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Polyethylene Glycols/*pharmacology/therapeutic use
MH  - T-Lymphocytes/*drug effects/immunology
EDAT- 2004/10/16 09:00
MHDA- 2010/06/04 06:00
CRDT- 2004/10/16 09:00
PHST- 2004/10/16 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
PHST- 2004/10/16 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Ke Za Zhi. 2004 Sep;42(9):684-7.