PMID- 15482769
OWN - NLM
STAT- MEDLINE
DCOM- 20041123
LR  - 20191210
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 82
IP  - 4
DP  - 2004 Oct
TI  - Fluorescence in situ hybridization analysis of human oocytes: advantages of a 
      double-labeling procedure.
PG  - 919-22
AB  - OBJECTIVE: To improve the fluorescence in situ hybridization (FISH) chromosomal 
      analysis of human oocytes and first polar bodies. DESIGN: In situ chromosomal 
      identification on isolated cells, with combinations of centromeric (or 
      locus-specific) probes and whole-chromosome painting probes for chromosomes 9, 
      13, 16, 18, 21, and X. SETTING: Montpellier University Hospital. PATIENT(S): 
      Women participating in an IVF program. INTERVENTION(S): Fifty-four in vitro 
      unfertilized oocytes were fixed on slides, and simple or double FISH labeling 
      procedures were performed on preparations. MAIN OUTCOME MEASURE(S): Simultaneous 
      in situ visualization of specific domains and chromosome arms of each targeted 
      chromosome. RESULT(S): Eight chromosomal abnormalities were identified, including 
      two hyperhaploidies, three cases of extra single chromatid, and three cases of 
      balanced separation of sister chromatids. Also, the double-labeling procedure 
      allowed the avoidance of five interpretation errors, owing to additional 
      artefactual signals. CONCLUSION(S): By ensuring precise identification of both 
      chromosomes and single chromatids, the FISH double-labeling procedure limits the 
      risk of erroneous interpretation and allows a more accurate cytogenetic analysis 
      of human oocytes.
FAU - Pellestor, Franck
AU  - Pellestor F
AD  - CNRS-UPR 1142, Montpellier, France. Franck.Pellestor@igh.cnrs.fr
FAU - Anahory, Tal
AU  - Anahory T
FAU - Andreo, Brigitte
AU  - Andreo B
FAU - Regnier-Vigouroux, Gilles
AU  - Regnier-Vigouroux G
FAU - Soulie, Jean Pierre
AU  - Soulie JP
FAU - Baudouin, Magalie
AU  - Baudouin M
FAU - Demaille, Jacques
AU  - Demaille J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Human/*genetics
MH  - Female
MH  - Fluorescent Dyes/pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oocytes/*ultrastructure
EDAT- 2004/10/16 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/16 09:00
PHST- 2003/06/12 00:00 [received]
PHST- 2004/03/03 00:00 [revised]
PHST- 2004/03/03 00:00 [accepted]
PHST- 2004/10/16 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/16 09:00 [entrez]
AID - S0015-0282(04)01279-8 [pii]
AID - 10.1016/j.fertnstert.2004.03.050 [doi]
PST - ppublish
SO  - Fertil Steril. 2004 Oct;82(4):919-22. doi: 10.1016/j.fertnstert.2004.03.050.