PMID- 15483336
OWN - NLM
STAT- MEDLINE
DCOM- 20050307
LR  - 20181130
IS  - 1011-8934 (Print)
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Linking)
VI  - 19
IP  - 5
DP  - 2004 Oct
TI  - Effects of cytokine milieu secreted by BCG-treated dendritic cells on 
      allergen-specific Th immune response.
PG  - 640-6
AB  - Bacillus Calmette-Guerin (BCG) is reported to suppress Th2 response and asthmatic 
      reaction. Dendritic cells (DCs), the major antigen-presenting cells, infections 
      with BCG are known to result in inducing various cytokines. Thus, DCs are likely 
      to play a role in the effects of BCG on asthma. This study aims at investigating 
      that cytokine milieu secreted by BCG-treated DCs directly enhances 
      allergen-specific Th1 response and/or suppresses Th2 response in allergic asthma. 
      DCs and CD3+ T cells were generated from Dermatophagoides farinae-sensitive 
      asthmatics. DCs were cultured with and without BCG and subjected to flow 
      cytometric analysis. IL-12 and IL-10 were determined from the culture 
      supernatants. Some DCs were cocultured with T cells in the presence of D. farinae 
      extracts after adding the culture supernatants from BCG-treated DCs, and IL-5 and 
      IFN-gamma were determined. BCG-treated DCs enhanced significantly the expressions 
      of CD80, CD86, and CD40, and the productions of IL-12 and IL-10. Addition of 
      culture supernatants from BCG-treated DCs up-regulated production of IFN-gamma by 
      T cells stimulated by DCs and D. farinae extracts (p<0.05), but did not 
      down-regulate production of IL-5 (p>0.05). The cytokine milieu secreted by 
      BCG-treated DCs directly enhanced allergen-specific Th1 response, although did 
      not suppress Th2 response.
FAU - Koh, Youngil I
AU  - Koh YI
AD  - Chonnam National University Medical School and Research Institute of Medical 
      Science, Gwangju, Korea. yikoh@chonnam.ac.kr.
FAU - Choi, Inseon S
AU  - Choi IS
FAU - Lee, Je-Jung
AU  - Lee JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-5)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, Dermatophagoides/*immunology
MH  - Asthma/*immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Culture Media
MH  - Cytokines/*immunology/metabolism
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Interferon-gamma/immunology/metabolism
MH  - Interleukin-10/immunology/metabolism
MH  - Interleukin-12/immunology/metabolism
MH  - Interleukin-5/immunology/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Mycobacterium bovis/*immunology
MH  - Th2 Cells/cytology/immunology/metabolism
MH  - Up-Regulation/immunology
PMC - PMC2816323
EDAT- 2004/10/16 09:00
MHDA- 2005/03/08 09:00
PMCR- 2004/10/01
CRDT- 2004/10/16 09:00
PHST- 2004/10/16 09:00 [pubmed]
PHST- 2005/03/08 09:00 [medline]
PHST- 2004/10/16 09:00 [entrez]
PHST- 2004/10/01 00:00 [pmc-release]
AID - 200410640 [pii]
AID - 10.3346/jkms.2004.19.5.640 [doi]
PST - ppublish
SO  - J Korean Med Sci. 2004 Oct;19(5):640-6. doi: 10.3346/jkms.2004.19.5.640.