PMID- 15485475
OWN - NLM
STAT- MEDLINE
DCOM- 20041130
LR  - 20131121
IS  - 0954-6820 (Print)
IS  - 0954-6820 (Linking)
VI  - 256
IP  - 5
DP  - 2004 Nov
TI  - Impairment of coronary circulation by acute hyperhomocysteinaemia and reversal by 
      antioxidant vitamins.
PG  - 398-405
AB  - OBJECTIVE: To evaluate the effect of acute hyperhomocysteinaemia with and without 
      antioxidant vitamins pretreatment on coronary circulation and circulating 
      chemokine levels. DESIGN: Observer-blinded, randomized crossover study. SETTING: 
      This study was conducted at a university hospital and at a general hospital in 
      Italy. SUBJECTS: Sixteen healthy hospital staff volunteers (nine men, seven 
      women), aged 26-40 years. INTERVENTIONS: Subjects were given each three loads in 
      random order at 1-week intervals: oral methionine, 100 mg kg(-1) in fruit juice; 
      the same methionine load immediately following ingestion of antioxidant vitamin 
      E, 800 IU, and ascorbic acid, 1000 mg; and methionine-free fruit juice (placebo). 
      MAIN OUTCOME MEASURES: Coronary flow velocity reserve (CFVR), assessed by 
      noninvasive transthoracic Doppler echocardiography, blood pressure, heart rate, 
      lipid and glucose, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 
      (IL-8) parameters evaluated at baseline and 4 h following ingestion of the loads. 
      RESULTS: The oral methionine load increased plasma homocysteine from 12.8 +/- 1.8 
      to 33.3 +/- 3.4 micromol L(-1) at 4 h (P < 0.001). A similar increase was 
      observed with same load plus vitamins (P < 0.001) but not with placebo (P = 
      0.14). Circulating MCP-1 and IL-8 levels rose after the methionine load (P < 
      0.001), but not after placebo or methionine plus vitamins. The methionine load 
      significantly reduced CFVR (decrease, 26 +/- 8.2%; P < 0.001). The methionine 
      load with ingestion of vitamins partially prevented the impairment of CFVR 
      (decrease, 11 +/- 4%; P < 0.001). CONCLUSION: Our data suggest that acute 
      hyperhomocysteinaemia reduces CFVR and increases plasma MCP-1 and IL-8 levels in 
      healthy subjects. Pretreatment with antioxidant vitamin E and ascorbic acid 
      prevents the effects of hyperhomocysteinaemia, suggesting an oxidative mechanism.
FAU - Coppola, A
AU  - Coppola A
AD  - Department of Geriatrics and Metabolic Diseases, Second University of Naples, 
      Naples, Italy. toncop@tiscali.it
FAU - Astarita, C
AU  - Astarita C
FAU - Liguori, E
AU  - Liguori E
FAU - Fontana, D
AU  - Fontana D
FAU - Oliviero, M
AU  - Oliviero M
FAU - Esposito, K
AU  - Esposito K
FAU - Coppola, L
AU  - Coppola L
FAU - Giugliano, D
AU  - Giugliano D
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - England
TA  - J Intern Med
JT  - Journal of internal medicine
JID - 8904841
RN  - 0 (Antioxidants)
RN  - 0 (Cytokines)
RN  - 0 (Vitamins)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Adult
MH  - Antioxidants/*therapeutic use
MH  - Coronary Circulation/*physiology
MH  - Coronary Disease/*etiology/prevention & control
MH  - Cross-Over Studies
MH  - Cytokines/metabolism
MH  - Female
MH  - Humans
MH  - Hyperhomocysteinemia/*physiopathology
MH  - Male
MH  - Methionine/therapeutic use
MH  - Vitamins/*therapeutic use
EDAT- 2004/10/16 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/16 09:00
PHST- 2004/10/16 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/16 09:00 [entrez]
AID - JIM1389 [pii]
AID - 10.1111/j.1365-2796.2004.01389.x [doi]
PST - ppublish
SO  - J Intern Med. 2004 Nov;256(5):398-405. doi: 10.1111/j.1365-2796.2004.01389.x.