PMID- 15487499 OWN - NLM STAT- MEDLINE DCOM- 20041103 LR - 20061115 IS - 0379-4172 (Print) IS - 0379-4172 (Linking) VI - 31 IP - 4 DP - 2004 Apr TI - Comparative physical localization of maize mir1 gene in Zea mays L. and Coix lacryma-jobi L. PG - 335-9 AB - The maize gene mir1 encoded a cystein proteinase which is resistant to fall armyworm. Previously, RFLP map indicated that the mir1 was mapped on chromosome 6. However, physical location of the mir1 gene on chromosome 6 has not been reported. In this study, the mir1 gene was physically located on the short arm of metaphase and pachytene chromosome 6 by dual-color fluorescence in situ hybridization (FISH) with 45S rDNA as a reference marker. The results of Southern blotting suggested that there were sequences homologous to mir1 in Coix lacryma-jobi L. genome. Then, the sequences were mapped on the distal region of long arm of chromosome 7 in C. lacryma-jobi by FISH. The percent distance from the signal site to centromere was 73.33 +/- 0.15. FAU - Han, Yong-Hua AU - Han YH AD - The Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China. hanyhzhou@sohu.com FAU - Wang, Xiao-Lan AU - Wang XL FAU - Liu, Li-Hua AU - Liu LH FAU - Song, Yun-Chuno AU - Song YC LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Yi Chuan Xue Bao JT - Yi chuan xue bao = Acta genetica Sinica JID - 7900784 SB - IM MH - Coix/*genetics MH - *Genes, Plant MH - In Situ Hybridization, Fluorescence MH - Nucleolus Organizer Region MH - *Physical Chromosome Mapping MH - Zea mays/*genetics EDAT- 2004/10/19 09:00 MHDA- 2004/11/04 09:00 CRDT- 2004/10/19 09:00 PHST- 2004/10/19 09:00 [pubmed] PHST- 2004/11/04 09:00 [medline] PHST- 2004/10/19 09:00 [entrez] PST - ppublish SO - Yi Chuan Xue Bao. 2004 Apr;31(4):335-9.