PMID- 15489342
OWN - NLM
STAT- MEDLINE
DCOM- 20041116
LR  - 20181113
IS  - 1088-9051 (Print)
IS  - 1088-9051 (Linking)
VI  - 14
IP  - 10B
DP  - 2004 Oct
TI  - The Pseudomonas aeruginosa PA01 gene collection.
PG  - 2190-200
AB  - Pseudomonas aeruginosa, a common inhabitant of soil and water, is an 
      opportunistic pathogen of growing clinical relevance. Its genome, one of the 
      largest among bacteria [5570 open reading frames (ORFs)] approaches that of 
      simple eukaryotes. We have constructed a comprehensive gene collection for this 
      organism utilizing the annotated genome of P. aeruginosa PA01 and a highly 
      automated and laboratory information management system (LIMS)-supported 
      production line. All the individual ORFs have been successfully PCR-amplified and 
      cloned into a recombination-based cloning system. We have isolated and archived 
      four independent isolates of each individual ORF. Full sequence analysis of the 
      first isolate for one-third of the ORFs in the collection has been completed. We 
      used two sets of genes from this repository for high-throughput expression and 
      purification of recombinant proteins in different systems. The purified proteins 
      have been used to set up biochemical and immunological assays directed towards 
      characterization of histidine kinases and identification of bacterial proteins 
      involved in the immune response of cystic fibrosis patients. This gene repository 
      provides a powerful tool for proteome- and genome-scale research of this 
      organism, and the strategies adopted to generate this repository serve as a model 
      for building clone sets for other bacteria.
FAU - Labaer, Joshua
AU  - Labaer J
AD  - Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical 
      School, Institute of Proteomics, Cambridge, Massachusetts 02141, USA.
FAU - Qiu, Qingqing
AU  - Qiu Q
FAU - Anumanthan, Anukanth
AU  - Anumanthan A
FAU - Mar, Wenhong
AU  - Mar W
FAU - Zuo, Dongmei
AU  - Zuo D
FAU - Murthy, T V S
AU  - Murthy TV
FAU - Taycher, Helen
AU  - Taycher H
FAU - Halleck, Allison
AU  - Halleck A
FAU - Hainsworth, Eugenie
AU  - Hainsworth E
FAU - Lory, Stephen
AU  - Lory S
FAU - Brizuela, Leonardo
AU  - Brizuela L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genome Res
JT  - Genome research
JID - 9518021
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - *Bacterial Proteins/genetics/metabolism
MH  - Cloning, Molecular
MH  - *Computational Biology/methods
MH  - DNA, Bacterial
MH  - DNA, Complementary/genetics/metabolism
MH  - Gene Expression
MH  - Genes, Bacterial/*physiology
MH  - Genetic Vectors
MH  - *Genome, Bacterial
MH  - Open Reading Frames/*physiology
MH  - Polymerase Chain Reaction
MH  - Pseudomonas aeruginosa/*chemistry/*genetics
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Analysis, DNA
PMC - PMC528936
EDAT- 2004/10/19 09:00
MHDA- 2004/11/17 09:00
PMCR- 2005/04/01
CRDT- 2004/10/19 09:00
PHST- 2004/10/19 09:00 [pubmed]
PHST- 2004/11/17 09:00 [medline]
PHST- 2004/10/19 09:00 [entrez]
PHST- 2005/04/01 00:00 [pmc-release]
AID - 14/10b/2190 [pii]
AID - 0142190 [pii]
AID - 10.1101/gr.2482804 [doi]
PST - ppublish
SO  - Genome Res. 2004 Oct;14(10B):2190-200. doi: 10.1101/gr.2482804.