PMID- 15490558
OWN - NLM
STAT- MEDLINE
DCOM- 20041123
LR  - 20131121
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 27
IP  - 5
DP  - 2004 Sep
TI  - The effect of nucleobase-specific fluorescence quenching on in situ hybridization 
      with rRNA-targeted oligonucleotide probes.
PG  - 565-72
AB  - Oligonucleotide probes labeled with fluorescent dyes are used in a variety of in 
      situ applications to detect specific DNA or RNA molecules. It has been described 
      that probe fluorescence might be quenched upon hybridization in a sequence 
      specific way. Here, a set of 17 oligonuleotides labeled with 6-carboxyfluorescein 
      was used to examine the relevance of nucleotide specific quenching for 
      fluorescence in situ hybridization (FISH) to whole fixed bacterial cells. Probes 
      quenched upon hybridization to a guanine-rich region of purified RNA in solution 
      were not quenched upon FISH. Among other factors the high protein concentration 
      within cells may prevent quenching of probe fluorescence in situ.
FAU - Behrens, Sebastian
AU  - Behrens S
AD  - Max Planck Institute of Marine Microbiology, Bremen, Germany.
FAU - Fuchs, Bernhard M
AU  - Fuchs BM
FAU - Amann, Rudolf
AU  - Amann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (Fluoresceins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal)
RN  - 3301-79-9 (6-carboxyfluorescein)
RN  - 5Z93L87A1R (Guanine)
SB  - IM
MH  - Base Sequence
MH  - Escherichia coli
MH  - Fluoresceins/chemistry
MH  - Fluorescence
MH  - Guanine/chemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - *Oligonucleotide Probes
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal/*analysis
MH  - Sensitivity and Specificity
EDAT- 2004/10/20 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/20 09:00
PHST- 2004/10/20 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/20 09:00 [entrez]
AID - S0723-2020(05)70295-2 [pii]
AID - 10.1078/0723202041748136 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2004 Sep;27(5):565-72. doi: 10.1078/0723202041748136.