PMID- 15492319
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20191210
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 110
IP  - 18
DP  - 2004 Nov 2
TI  - Cellular physiology of cholesterol efflux in vascular endothelial cells.
PG  - 2881-8
AB  - BACKGROUND: Of the cells that compose the atherosclerotic plaque, vascular 
      endothelial cells are the most resistant to cholesterol accumulation. Cholesterol 
      efflux pathways may play an important role in endothelial cholesterol 
      homeostasis. METHODS AND RESULTS: We examined the global genetic response of 
      endothelial cells to cholesterol and in particular the contribution of the 
      cholesterol efflux proteins ATP-binding cassette transporter A1 (ABCA1), 
      ATP-binding cassette transporter G1 (ABCG1), and scavenger receptor B-I (SR-BI) 
      to endothelial cell cholesterol efflux. The ABCG1 gene is induced in endothelial 
      cells by cholesterol, whereas ABCA1 is not. Using specific chemical inhibitors of 
      ABC transporters and SR-BI, we have shown that neither ABC transporters nor SR-BI 
      is required for apolipoprotein A-1-mediated endothelial cholesterol efflux. 
      CONCLUSIONS: Endothelial cells may use nontraditional pathways for cholesterol 
      efflux.
FAU - O'Connell, Brian J
AU  - O'Connell BJ
AD  - Cardiovascular Genetics Laboratory, McGill University Health Centre, Royal 
      Victoria Hospital, Montreal, Quebec, Canada.
FAU - Denis, Maxime
AU  - Denis M
FAU - Genest, Jacques
AU  - Genest J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041018
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Apolipoprotein A-I)
RN  - 0 (CD36 Antigens)
RN  - 0 (Hydroxycholesterols)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Lipoproteins, HDL3)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Scavenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 17711-16-9 (22-hydroxycholesterol)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - SX6K58TVWC (Glyburide)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - ATP Binding Cassette Transporter 1
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/antagonists & inhibitors/genetics/metabolism
MH  - Animals
MH  - Aorta
MH  - Apolipoprotein A-I/pharmacology
MH  - CD36 Antigens
MH  - COS Cells
MH  - Cattle
MH  - Cells, Cultured/drug effects/metabolism
MH  - Chlorocebus aethiops
MH  - Cholesterol/*metabolism/pharmacology
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Endothelium, Vascular/cytology/*physiology
MH  - Fibroblasts/drug effects/metabolism/pathology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Glyburide/pharmacology
MH  - Humans
MH  - Hydroxycholesterols/pharmacology
MH  - Lipoproteins, HDL/pharmacology
MH  - Lipoproteins, HDL3
MH  - Oligonucleotide Array Sequence Analysis
MH  - Receptors, Immunologic/antagonists & inhibitors/genetics/metabolism
MH  - Receptors, Scavenger
MH  - Recombinant Fusion Proteins/physiology
MH  - Serum Albumin, Bovine/pharmacology
MH  - Tangier Disease/genetics/pathology
MH  - Transfection
MH  - Umbilical Veins
EDAT- 2004/10/20 09:00
MHDA- 2005/10/01 09:00
CRDT- 2004/10/20 09:00
PHST- 2004/10/20 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2004/10/20 09:00 [entrez]
AID - 01.CIR.0000146333.20727.2B [pii]
AID - 10.1161/01.CIR.0000146333.20727.2B [doi]
PST - ppublish
SO  - Circulation. 2004 Nov 2;110(18):2881-8. doi: 10.1161/01.CIR.0000146333.20727.2B. 
      Epub 2004 Oct 18.