PMID- 15493453
OWN - NLM
STAT- MEDLINE
DCOM- 20050125
LR  - 20190922
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 38
IP  - 8
DP  - 2004 Aug
TI  - Anti-inflammatory effects of Melaleuca alternifolia essential oil on human 
      polymorphonuclear neutrophils and monocytes.
PG  - 805-11
AB  - OBJECTIVE AND DESIGN: The fungicidal and bactericidal actions of the essential 
      oil (EO) of Melaleuca alternifolia seem well established, but their 
      anti-inflammatory and anti-oxidative effects remain unclear. In this study, we 
      investigated in vitro the possible role of whole M. alternifolia EO as a 
      modulator of the oxidative response, i.e. reactive oxygen species (ROS) 
      production, of leukocytes (monocytes and polymorphonuclear neutrophils (PMNs)) in 
      humans. METHODS: Whole blood leukocytes from healthy human volunteers (n = 7), 
      isolated from erythrocytes by haemolytic shock, were incubated for 30 min with M. 
      alternifolia EO (0-0.1%) to determine their ROS production by flow cytometry with 
      or without stimulation of cells. We compared the effects of 3 different 
      stimulating agents acting differently on transductional pathways to stimulate the 
      ROS production: a phorbol ester (PMA), formyl-methionyl-leucyl-phenylalanine 
      (fMLP) and opsonised zymosan (OZ). RESULTS: As attested by the Kruskall-Wallis 
      test, M. alternifolia EO at 0.1% directly stimulated ROS production by PMNs (x 
      8.7 vs. 0% EO, p < 0.05) and increased the intracellular ROS produced by 
      monocytes. Whichever the stimulating agent used (PMA, fMLP or OZ), M. 
      alternifolia EO decreased the intracellular ROS production at the dilution of 
      0.1% by PMNs and monocytes, more so with PMNs. CONCLUSION: M. alternifolia EO may 
      be both a direct active mediator of the bactericidal action of the circulating 
      leukocytes and may be efficient in protecting the organism from an excess of ROS, 
      through an anti-oxidant and radical scavenging activity.
FAU - Caldefie-Chezet, F
AU  - Caldefie-Chezet F
AD  - Laboratoire de Botanique, Cryptogamie et Microbiologie, Faculte des Sciences, 
      Clermont-Ferrand, France. florence.caldefie-chezet@u-clermont1.fr
FAU - Guerry, M
AU  - Guerry M
FAU - Chalchat, J C
AU  - Chalchat JC
FAU - Fusillier, C
AU  - Fusillier C
FAU - Vasson, M P
AU  - Vasson MP
FAU - Guillot, J
AU  - Guillot J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 68647-73-4 (Tea Tree Oil)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Melaleuca/chemistry
MH  - Monocytes/*drug effects/metabolism
MH  - Neutrophils/*drug effects/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Tea Tree Oil/chemistry/*pharmacology
EDAT- 2004/10/21 09:00
MHDA- 2005/01/26 09:00
CRDT- 2004/10/21 09:00
PHST- 2004/10/21 09:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/10/21 09:00 [entrez]
AID - 10.1080/1071576042000220247 [doi]
PST - ppublish
SO  - Free Radic Res. 2004 Aug;38(8):805-11. doi: 10.1080/1071576042000220247.