PMID- 15494131
OWN - NLM
STAT- MEDLINE
DCOM- 20050411
LR  - 20061115
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 334
IP  - 2
DP  - 2004 Nov 15
TI  - Characterization of ionizing radiation-induced ring chromosomes by atomic force 
      microscopy.
PG  - 251-6
AB  - We applied atomic force microscopy (AFM) to the structural analysis of 
      radiation-induced ring chromosomes. Constrictions observed on the metaphase ring 
      chromosome were found to correspond to the centromere regions of the ring 
      chromosome in comparison with the AFM image of the centromere of rod chromosomes 
      and with the fluorescence in situ hybridization (FISH) technique. Section 
      analysis by AFM revealed that some ring-like chromosome fragments and ring-like 
      chromatid fragments were thicker than standard chromosomes or chromatids, 
      suggesting that they were ring chromosomes viewed edge on. Topographic analysis 
      by AFM makes it possible to distinguish a ring viewed edge on that is difficult 
      to recognize as a ring by light microscopy and to discriminate between a centric 
      ring chromosome and an acentric ring chromosome using the same slides prepared 
      for light microscopy.
FAU - Murakami, Masahiro
AU  - Murakami M
AD  - Radiation Hazards Research Group, Research Center for Radiation Safety, National 
      Institute of Radiological Sciences, 9-1, Anagawa-4-chome, Inage-ku, Chiba-shi 
      263-8555, Japan. drhmmura@nirs.go.jp
FAU - Kanda, Reiko
AU  - Kanda R
FAU - Minamihisamatsu, Masako
AU  - Minamihisamatsu M
FAU - Hayata, Isamu
AU  - Hayata I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
SB  - IM
MH  - Centromere/radiation effects/ultrastructure
MH  - Chromosomes, Human/*radiation effects/*ultrastructure
MH  - Female
MH  - Humans
MH  - Metaphase/radiation effects
MH  - *Microscopy, Atomic Force
MH  - *Radiation, Ionizing
MH  - *Ring Chromosomes
EDAT- 2004/10/21 09:00
MHDA- 2005/04/12 09:00
CRDT- 2004/10/21 09:00
PHST- 2004/05/26 00:00 [received]
PHST- 2004/10/21 09:00 [pubmed]
PHST- 2005/04/12 09:00 [medline]
PHST- 2004/10/21 09:00 [entrez]
AID - S0003-2697(04)00617-7 [pii]
AID - 10.1016/j.ab.2004.07.023 [doi]
PST - ppublish
SO  - Anal Biochem. 2004 Nov 15;334(2):251-6. doi: 10.1016/j.ab.2004.07.023.