PMID- 15494434
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 105
IP  - 9
DP  - 2005 May 1
TI  - Dendritic-cell activation by secretory phospholipase A2.
PG  - 3583-7
AB  - Dendritic cells (DCs), also referred to as the sentinels of the immune system, 
      induce and coordinate important functions of immune surveillance. DCs acquire 
      immunity-initiating capacity only after a process of maturation usually induced 
      by ligands that bind to members of the tumor necrosis factor (TNF) or toll-like 
      receptor families. Secretory phospholipase A2 (sPLA2), which hydrolyzes the sn-2 
      ester bond of glycerophospholipids, regulates a variety of cellular functions 
      including migration of endothelial cells and neurite outgrowth. In the present 
      study we investigated the role of sPLA2 in DC biology. We report that human 
      monocyte-derived DC cultures lack sPLA2 activity but respond to exogenous sPLA2. 
      sPLA2 alone and in cooperation with TNF-alpha and interleukin 1 beta (IL-1beta) 
      induced fatty acid release from DC membranes, which was accompanied by 
      upregulation of surface markers and by an increase in the migratory and 
      immunostimulatory capacity of the DCs. Our findings indicate that secreted 
      enzymes such as sPLA2 can contribute to DC maturation and emphasize the role of 
      lipid mediators in the regulation of immune responses. This observation may also 
      have implications for DC-based vaccine development.
FAU - Ramoner, Reinhold
AU  - Ramoner R
AD  - Department of Urology and Kompetenzzentrum Medizin Tirol (KMT), Medical 
      University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.
FAU - Putz, Thomas
AU  - Putz T
FAU - Gander, Hubert
AU  - Gander H
FAU - Rahm, Andrea
AU  - Rahm A
FAU - Bartsch, Georg
AU  - Bartsch G
FAU - Schaber, Claudia
AU  - Schaber C
FAU - Thurnher, Martin
AU  - Thurnher M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041019
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, Surface)
RN  - 0 (Bee Venoms)
RN  - 0 (Fatty Acids)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
MH  - Animals
MH  - Antigens, Surface
MH  - Bee Venoms/enzymology
MH  - Bees
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*immunology/metabolism
MH  - Fatty Acids/metabolism
MH  - Humans
MH  - Immunity
MH  - Interleukin-1/pharmacology
MH  - Phospholipases A/metabolism/*pharmacology
MH  - Phospholipases A2
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Up-Regulation
EDAT- 2004/10/21 09:00
MHDA- 2005/06/09 09:00
CRDT- 2004/10/21 09:00
PHST- 2004/10/21 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2004/10/21 09:00 [entrez]
AID - S0006-4971(20)45553-3 [pii]
AID - 10.1182/blood-2004-08-3001 [doi]
PST - ppublish
SO  - Blood. 2005 May 1;105(9):3583-7. doi: 10.1182/blood-2004-08-3001. Epub 2004 Oct 
      19.