PMID- 15496670
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20220309
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 24
IP  - 42
DP  - 2004 Oct 20
TI  - Action potential backpropagation and multiglomerular signaling in the rat 
      vomeronasal system.
PG  - 9341-52
AB  - In the accessory olfactory bulb (AOB), sensory neurons expressing a given 
      vomeronasal receptor (VR) gene send divergent projections to many glomeruli, and 
      second-order neurons (mitral cells) link to multiple glomeruli via branched 
      primary dendrites. We used calcium imaging and paired somadendritic patch-clamp 
      recording to track backpropagated action potentials (APs) in rat AOB primary 
      dendrites. In cells loaded with 150 microm Calcium Orange, somatic spikes 
      elicited fluorescence transients over the entire primary dendritic tree, and the 
      relative fluorescence increment DeltaF/F(0) increased along all branches from 
      soma to glomeruli. Backpropagation was reliant on Na+ channels: in 1 microm TTX, 
      somatic AP commands evoked dendritic Ca2+ transients that declined steeply with 
      distance. In paired soma- dendritic whole-cell recordings, backpropagated APs 
      were unattenuated up to approximately 200 microm from the soma, whereas 
      subthreshold voltage transients decayed markedly. Computational modeling 
      indicated that the large distal Ca2+ transients are consistent with active, not 
      passive, backpropagation. Genetic tracing in the AOB has suggested homotypic 
      connectivity with individual mitral cell dendritic arbors projecting only to 
      glomeruli targeted by sensory neurons expressing the same VR gene. 
      Non-decremental, non-dichotomous backpropagation in AOB primary dendrites ensures 
      fast, reliable communication between mitral cells and their homotypic glomeruli, 
      binding them into functional modules in accordance with their VR-coded inputs.
FAU - Ma, Jie
AU  - Ma J
AD  - Monell Chemical Senses Center, Philadelphia, Pennsylvania 19104-3308, USA.
FAU - Lowe, Graeme
AU  - Lowe G
LA  - eng
GR  - R01 DC004208/DC/NIDCD NIH HHS/United States
GR  - DC04208/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Calcium/physiology
MH  - Dendrites/*physiology
MH  - In Vitro Techniques
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Models, Neurological
MH  - Nerve Endings/*physiology
MH  - Neural Conduction/physiology
MH  - Olfactory Bulb/cytology/*physiology
MH  - Patch-Clamp Techniques
MH  - Potassium/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/physiology
MH  - Vomeronasal Organ/*physiology
PMC - PMC6730108
EDAT- 2004/10/22 09:00
MHDA- 2005/04/29 09:00
PMCR- 2005/04/20
CRDT- 2004/10/22 09:00
PHST- 2004/10/22 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2004/10/22 09:00 [entrez]
PHST- 2005/04/20 00:00 [pmc-release]
AID - 24/42/9341 [pii]
AID - 10.1523/JNEUROSCI.1782-04.2004 [doi]
PST - ppublish
SO  - J Neurosci. 2004 Oct 20;24(42):9341-52. doi: 10.1523/JNEUROSCI.1782-04.2004.