PMID- 15497144
OWN - NLM
STAT- MEDLINE
DCOM- 20050331
LR  - 20041027
IS  - 1527-6465 (Print)
IS  - 1527-6465 (Linking)
VI  - 10
IP  - 11
DP  - 2004 Nov
TI  - Detection of recipient's cells in liver graft using antibodies to mismatched HLA 
      class I antigens.
PG  - 1406-14
AB  - Engraftment by recipient's (R) cells has been already demonstrated in gender 
      mismatched liver grafts using fluorescence in situ hybridization (FISH), with 
      contrasting results concerning epithelial cells. Mismatch for human leukocyte 
      antigen (HLA) class I (HLA-I) is quite common in patients with orthotopic liver 
      transplantation (OLT). We thus aimed to assess whether monoclonal antibodies 
      (MoAbs), currently employed in the HLA typing process, could be used to study the 
      dynamics of R cells in liver grafts. A total of 50 frozen liver biopsies from 37 
      patients receiving a HLA mismatch liver were tested. Biopsies were obtained from 
      3 days to more than 360 days after OLT. Frozen sections of graft biopsies were 
      stained using an immunoperoxidase technique with the proper MoAbs. In selected 
      cases, a double immunofluorescence was also performed. Circulating R blood cells 
      and sinusoidal cells were occasionally observed in liver biopsies obtained within 
      10 days after OLT and were commonly detected after 1 month. The number of 
      sinusoidal cells continued to increase up to 6 months, as shown on serial 
      biopsies. On the whole, R blood cells and R sinusoidal cells were detected in 86% 
      and 82% of the biopsies, respectively. R hepatocytes and biliary cells were 
      detected after 40 and 60 days after OLT, respectively, in 14% (hepatocytes), 8% 
      (bile ducts), and 12% (proliferating bile ducts) of the biopsies. R hepatocytes 
      presented as single cells or groups of few cells; their number was lower than 1% 
      and apparently did not increase with time after OLT. In conclusion, it is 
      possible to detect R cells in liver graft using MoAbs to specific mismatched 
      HLA-I alleles. R sinusoidal cells start to appear after 10 days and are commonly 
      observed after 1 month; bile duct cells and hepatocytes appear later and their 
      number does not increase with time. Engraftment by R epithelial cells seems to be 
      less important than previously reported.
FAU - Grassi, Alberto
AU  - Grassi A
AD  - University of Bologna-S. Orsola Hospital, Bologna, Italy. albgrassi@yahoo.com
FAU - Susca, Micaela
AU  - Susca M
FAU - Ravaioli, Matteo
AU  - Ravaioli M
FAU - Grazi, Gian Luca
AU  - Grazi GL
FAU - D'Errico, Antonia
AU  - D'Errico A
FAU - Bontadini, Andrea
AU  - Bontadini A
FAU - Zauli, Daniela
AU  - Zauli D
FAU - Pinna, Antonio
AU  - Pinna A
FAU - Bianchi, Francesco B
AU  - Bianchi FB
FAU - Ballardini, Giorgio
AU  - Ballardini G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Liver Transpl
JT  - Liver transplantation : official publication of the American Association for the 
      Study of Liver Diseases and the International Liver Transplantation Society
JID - 100909185
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Antibodies, Monoclonal/*immunology
MH  - Biopsy
MH  - HLA Antigens/*immunology
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Liver/*immunology/pathology
MH  - Liver Transplantation/*immunology
MH  - Transplants
EDAT- 2004/10/22 09:00
MHDA- 2005/04/01 09:00
CRDT- 2004/10/22 09:00
PHST- 2004/10/22 09:00 [pubmed]
PHST- 2005/04/01 09:00 [medline]
PHST- 2004/10/22 09:00 [entrez]
AID - 10.1002/lt.20242 [doi]
PST - ppublish
SO  - Liver Transpl. 2004 Nov;10(11):1406-14. doi: 10.1002/lt.20242.