PMID- 15498041
OWN - NLM
STAT- MEDLINE
DCOM- 20041214
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 138
IP  - 2
DP  - 2004 Nov
TI  - Mannan-binding lectin modulates the response to HSV-2 infection.
PG  - 304-11
AB  - Viruses have developed numerous strategies to escape recognition by the immune 
      system. However, some viruses such as herpes simplex virus-2 (HSV-2) are 
      recognized by initiators of the complement system, e.g. mannan-binding lectin 
      (MBL). To study the effects of MBL deficiency during viral infection we have 
      chosen a model of generalized HSV-2 infection. We infected MBL-A and MBL-C double 
      knock-out mice (DKO) with HSV-2 via the intraperitoneal (i.p.) route. DKO mice 
      cleared HSV-2 from the liver less efficiently than the comparable wild-type 
      animals. The impairment to effectively neutralize HSV-2 correlated with 
      compromised liver function as measured by increased plasma levels of 
      alanine-amino transferase. No differences in the viral burden were found in other 
      organs such as spleen or brain. Thus, MBL-mediated protection was limited to the 
      effects of preservation of liver homeostasis. Reconstitution with recombinant 
      human MBL before and during the HSV-2 infection dramatically lowered the viral 
      titres in the liver. Taken together, the data show that MBL modulates the 
      response to HSV-2 in mice by affecting neutralization of the virus. To analyse if 
      MBL plays a role in establishment and progression of human HSV-2 infection we 
      analysed MBL levels in the serum samples from asymptomatic (virus-exposed people 
      who have never displayed symptoms of HSV-2 infection) and symptomatic HSV-2 
      patients (people with recurrent HSV-2 infections). We found that the frequency of 
      the MBL deficiency (<100 ng/ml) was higher in the symptomatic group and 
      significantly different from that in the asymptomatic group (P = 0.0369). This 
      suggests that lack of MBL-mediated complement activation increases susceptibility 
      to viral infection.
FAU - Gadjeva, M
AU  - Gadjeva M
AD  - Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, 
      Denmark. mg@immunology.au.dk
FAU - Paludan, S R
AU  - Paludan SR
FAU - Thiel, S
AU  - Thiel S
FAU - Slavov, V
AU  - Slavov V
FAU - Ruseva, M
AU  - Ruseva M
FAU - Eriksson, K
AU  - Eriksson K
FAU - Lowhagen, G-B
AU  - Lowhagen GB
FAU - Shi, L
AU  - Shi L
FAU - Takahashi, K
AU  - Takahashi K
FAU - Ezekowitz, A
AU  - Ezekowitz A
FAU - Jensenius, J C
AU  - Jensenius JC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Mannose-Binding Lectin)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Brain/immunology
MH  - Female
MH  - Herpes Genitalis/immunology
MH  - Herpes Simplex/blood/*immunology
MH  - Herpesvirus 2, Human/*immunology
MH  - Homeostasis/immunology
MH  - Humans
MH  - Liver/immunology
MH  - Male
MH  - Mannose-Binding Lectin/blood/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Middle Aged
MH  - Recombinant Proteins/immunology
MH  - Recurrence
MH  - Spleen/immunology
MH  - Viral Load/methods
MH  - Viral Proteins/immunology
PMC - PMC1809223
EDAT- 2004/10/23 09:00
MHDA- 2004/12/16 09:00
PMCR- 2005/11/01
CRDT- 2004/10/23 09:00
PHST- 2004/10/23 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/23 09:00 [entrez]
PHST- 2005/11/01 00:00 [pmc-release]
AID - CEI2616 [pii]
AID - 10.1111/j.1365-2249.2004.02616.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2004 Nov;138(2):304-11. doi: 10.1111/j.1365-2249.2004.02616.x.