PMID- 15498383
OWN - NLM
STAT- MEDLINE
DCOM- 20041130
LR  - 20061115
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 117
IP  - 10
DP  - 2004 Oct
TI  - Induction of T-cell immunity against Epstein-Barr virus-associated tumors by 
      means of adenovirally transduced dendritic cells.
PG  - 1558-63
AB  - BACKGROUND: Dendritic cells (DCs) are the most powerful antigen-presenting cells 
      to induce specific T-cell immunity, which plays an important role in the body's 
      anti-tumor responses. In this study, we assessed the feasibility and efficacy of 
      inducing T-cell immunity against Epstein-Barr virus (EBV)-associated tumors in 
      vivo using dendritic cells transfected with EBV latent membrane 2A (LMP2A) 
      recombinant adenovirus. METHODS: Cytokine-activated bone marrow-derived DCs 
      transfected with EBV LMP2A recombinant adenovirus were infused into BALB/c mice. 
      Splenic cytotoxic T-cell responses were evaluated by cytotoxicity and 
      interferon-gamma production assays. in vivo immune protection was then assessed 
      in the mice tumor models implanted with tumor cells expressing EBV LMP2A. 
      RESULTS: DCs transfected with EBV LMP2A recombinant adenovirus could strongly 
      induce EBV LMP2A-specific cytotoxic T-cell responses and upregulate 
      interferon-gamma production in vivo. Vaccination using these DCs led to 
      prolongation of overall survival rates in the mice tumor models and retarded 
      tumor growth. CONCLUSIONS: The results suggest that DCs transfected with EBV 
      LMP2A recombinant adenovirus can serve as a feasible and effective tool for 
      eliciting LMP2A-specific cytotoxic T-cell responses against EBV LMP2A in vivo in 
      the treatment of EBV-associated tumors.
FAU - Sun, Hua
AU  - Sun H
AD  - Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 
      210029, China.
FAU - Yao, Kun
AU  - Yao K
FAU - Chen, Yun
AU  - Chen Y
FAU - Zhou, Feng
AU  - Zhou F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (EBV-associated membrane antigen, Epstein-Barr virus)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Herpesvirus 4, Human/*immunology
MH  - Immunotherapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasms, Experimental/*therapy
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transduction, Genetic
MH  - Vaccination
MH  - Viral Matrix Proteins/*immunology
EDAT- 2004/10/23 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/23 09:00
PHST- 2004/10/23 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/23 09:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2004 Oct;117(10):1558-63.