PMID- 15498848
OWN - NLM
STAT- MEDLINE
DCOM- 20050303
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 105
IP  - 4
DP  - 2005 Feb 15
TI  - Monocyte chemoattractant protein-1-induced angiogenesis is mediated by vascular 
      endothelial growth factor-A.
PG  - 1405-7
AB  - Monocyte chemoattractant protein-1 (MCP-1) has been recognized as an angiogenic 
      chemokine. In the present study, we investigated the detailed mechanism by which 
      MCP-1 induces angiogenesis. We found that MCP-1 up-regulated hypoxia-inducible 
      factor 1 alpha (HIF-1 alpha) gene expression in human aortic endothelial cells 
      (HAECs), which induced vascular endothelial growth factor-A(165) (VEGF-A(165)) 
      expression in the aortic wall and HAECs through activation of p42/44 
      mitogen-activated protein kinase (MAPK). In vivo angiogenesis assay using chick 
      chorioallantoic membrane (CAM) showed that MCP-1-induced angiogenesis was as 
      potent as that induced by VEGF-A(165) and completely inhibited by a VEGF 
      inhibitor, Flt(2-11). The inhibition of RhoA small G protein did not affect 
      MCP-1-induced VEGF-A(165) production and secretion but completely blocked both 
      MCP-1- and VEGF-A-induced new vessel formation, as determined by CAM assay. These 
      results suggest that MCP-1-induced angiogenesis is composed largely of 2 
      sequential steps: the induction of VEGF-A gene expression by MCP-1 and the 
      subsequent VEGF-A-induced angiogenesis.
FAU - Hong, Kyung Hee
AU  - Hong KH
AD  - Department of Cardiology, University of Ulsan College of Medicine, Asan Medical 
      Center, 388-1 Pungnap-2 dong Songpa-gu 138-736, Seoul, South Korea.
FAU - Ryu, Jewon
AU  - Ryu J
FAU - Han, Ki Hoon
AU  - Han KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041021
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Angiogenesis Inducing Agents/*administration & dosage
MH  - Animals
MH  - Aorta, Abdominal/physiology
MH  - Aorta, Thoracic/physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*administration & dosage/metabolism
MH  - Endothelium, Vascular/cytology/metabolism/physiology
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Male
MH  - Neovascularization, Physiologic/*physiology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transcription Factors/biosynthesis/genetics
MH  - Vascular Endothelial Growth Factor A/biosynthesis/genetics/*physiology
EDAT- 2004/10/23 09:00
MHDA- 2005/03/04 09:00
CRDT- 2004/10/23 09:00
PHST- 2004/10/23 09:00 [pubmed]
PHST- 2005/03/04 09:00 [medline]
PHST- 2004/10/23 09:00 [entrez]
AID - S0006-4971(20)45859-8 [pii]
AID - 10.1182/blood-2004-08-3178 [doi]
PST - ppublish
SO  - Blood. 2005 Feb 15;105(4):1405-7. doi: 10.1182/blood-2004-08-3178. Epub 2004 Oct 
      21.