PMID- 15499412
OWN - NLM
STAT- MEDLINE
DCOM- 20050331
LR  - 20220408
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 47
IP  - 5
DP  - 2004 Oct
TI  - Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH.
PG  - 979-87
AB  - Fluorescence in situ hybridization (FISH) is widely used in the physical mapping 
      of genes and chromosome landmarks in plants and animals. Bacterial artificial 
      chromosomes (BACs) contain large inserts, making them amenable for FISH mapping. 
      In our BAC-FISH experiments, we selected 56 restriction fragment length 
      polymorphism (RFLP)-locus-specific BAC clones from the libraries of Triticum 
      monococcum and Aegilops tauschii, which are the A- and D-genome donors of wheat 
      (Triticum aestivum, 2n = 6x = 42), respectively. The BAC clone 676D4 from the T. 
      monococcum library contains a dispersed repeat that preferentially hybridizes to 
      A-genome chromosomes, and two BAC clones, 9I10 and 9M13, from the Ae. tauschii 
      library contain a dispersed repeat that preferentially hybridizes to the D-genome 
      chromosomes. These repeats are useful in simultaneously discriminating the three 
      different genomes in hexaploid wheat, and in identifying intergenomic 
      translocations in wheat or between wheat and alien chromosomes. Sequencing 
      results show that both of these repeats are transposable elements, indicating the 
      importance of transposable elements, especially retrotransposons, in the genome 
      evolution of wheat.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Wheat Genetics Resources Center, Department of Plant Pathology, 4024 Throckmorton 
      Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.
FAU - Li, Wanlong
AU  - Li W
FAU - Friebe, Bernd
AU  - Friebe B
FAU - Gill, Bikram S
AU  - Gill BS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA Transposable Elements)
SB  - IM
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Chromosomes, Plant/*genetics
MH  - Cloning, Molecular
MH  - DNA Transposable Elements/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polyploidy
MH  - Triticum/*genetics
EDAT- 2004/10/23 09:00
MHDA- 2005/04/01 09:00
CRDT- 2004/10/23 09:00
PHST- 2004/10/23 09:00 [pubmed]
PHST- 2005/04/01 09:00 [medline]
PHST- 2004/10/23 09:00 [entrez]
AID - g04-042 [pii]
AID - 10.1139/g04-042 [doi]
PST - ppublish
SO  - Genome. 2004 Oct;47(5):979-87. doi: 10.1139/g04-042.