PMID- 15500769
OWN - NLM
STAT- MEDLINE
DCOM- 20061214
LR  - 20091119
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 40
IP  - 9
DP  - 2004 Sep
TI  - [Posterior vitreous detachment induced by intravitreal injection of the 
      combination of chondroitinase ABC and matrix metalloproteinase-3].
PG  - 625-31
AB  - OBJECTIVE: To investigate the occurrence of enzymatic induction of posterior 
      vitreous detachment (PVD) by the combination of Chondroitinase ABC (CA) and 
      matrix metalloproteinase-3 (MMP-3), so as to seek a noninvasive and effective 
      pharmacologic approach to facilitate and eventually replace the present 
      mechanical vitreous surgery. METHODS: Twenty-four pigmented rabbits were randomly 
      assigned to two groups of twelve each, the experimental group was treated with CA 
      (0.2 U) and MMP-3 (10 ng) combination, the control group was received equivalent 
      dose of balanced salt solution (BSS). Clinical examinations and 
      electroretinography were performed before and after injection. Over a different 
      period of time, the rabbits were euthanized and killed and their eyes were 
      examined histologically. RESULTS: The foci of partial synchisis and clinically 
      named PVD were recognized for the first time three days after injection. Complete 
      liquefaction was found in every eye of experimental group, and in which, 5/8 eyes 
      developed clinically detected PVD one week after injection Histologic section 
      showed PVD with various extent in 3/7, 7/7, 7/7 eyes of experimental group 30 
      minutes, 60 minutes and one week after injection respectively, and complete PVD 
      in 0/7, 1/7, 5/7 eyes of experimental group at the same periodic intervals as 
      above. By contrast, no vitreous liquefaction was found and only one eye showed 
      confined partial PVD in the control group. Clinic, electrophysiologic and 
      histologic evaluation in all rabbits revealed no evidence of ocular toxicity. 
      CONCLUSIONS: Vitreous 1iquefaction and PVD can be produced shortly after 
      intravitreal injection using a combination of CA and MMP-3, and the two enzymes 
      were cooperative. Synchisis and weakening of vitreoretinal adherence were almost 
      simultaneously. The dose of 0.2 U CA and 10 ng MMP-3 combination proved to be 
      safe and ideal selection to induce PVD.
FAU - Meng, Zi-jun
AU  - Meng ZJ
AD  - Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430032, China. 
      mongeon0319@hotmail.com
FAU - Zeng, Shui-qing
AU  - Zeng SQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Animals
MH  - Chondroitin ABC Lyase/adverse effects/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Matrix Metalloproteinase 3/adverse effects/*pharmacology
MH  - Rabbits
MH  - Vitreous Detachment/*chemically induced/pathology
EDAT- 2004/10/27 09:00
MHDA- 2006/12/15 09:00
CRDT- 2004/10/27 09:00
PHST- 2004/10/27 09:00 [pubmed]
PHST- 2006/12/15 09:00 [medline]
PHST- 2004/10/27 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2004 Sep;40(9):625-31.