PMID- 15500979
OWN - NLM
STAT- MEDLINE
DCOM- 20041217
LR  - 20121115
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 240
IP  - 1
DP  - 2004 Nov 1
TI  - Regulation system for protease production in Vibrio vulnificus.
PG  - 55-9
AB  - Vibrio vulnificus is a causative agent of serious food-borne diseases in humans 
      related to consumption of raw seafoods. This human pathogen secretes a 
      metalloprotease (VVP) that evokes enhancement of the vascular permeability and 
      disruption of the capillaries. Production of microbial proteases is generally 
      induced at early stationary phase of its growth. This cell density dependent 
      regulation of VVP production in V. vulnificus known to be the quorum-sensing. 
      When V. vulnificus was cultivated in Luria-Bertani (LB) medium, accumulation of 
      the autoinducer, the signal molecule operating the quorum-sensing system, was 
      detected. Moreover, expression of the vvp gene encoding VVP was found to be 
      closely related with expression of the luxS gene that encode the synthase of the 
      autoinducer precursor (luxS). These findings may indicate VVP production is 
      controlled by the quorum-sensing system in LB medium. Furthermore, this system 
      functioned more effectively at 26 degrees C than at 37 degrees C. When incubated 
      at 37 degrees C in human serum supplemented with ferric chloride, production of 
      VVP and expression of vvp increased in proportion to the concentration of ferric 
      ion; whereas, expression of luxS was not increased. This suggests that VVP 
      production in human serum containing ferric ion may be regulated mainly by the 
      system other than the quorum-sensing system.
FAU - Kawase, Tomoka
AU  - Kawase T
AD  - Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Okayama 
      700-8530, Japan. dnsl4321@cc.okayama-u.ac.jp
FAU - Miyoshi, Shin-ichi
AU  - Miyoshi S
FAU - Sultan, Zafar
AU  - Sultan Z
FAU - Shinoda, Sumio
AU  - Shinoda S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Bacterial Proteins)
RN  - 0 (Boric Acids)
RN  - 0 (Culture Media)
RN  - 0 (Ferric Compounds)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.- (vvP protein, Vibrio vulnificus)
RN  - EC 4.4.- (Carbon-Sulfur Lyases)
RN  - EC 4.4.1.21 (LuxS protein, Bacteria)
RN  - R57ZHV85D4 (boric acid)
SB  - IM
MH  - Bacterial Proteins/*genetics/*metabolism
MH  - Boric Acids/pharmacology
MH  - Carbon-Sulfur Lyases
MH  - Culture Media
MH  - Ferric Compounds/pharmacology
MH  - Gene Expression Regulation, Bacterial
MH  - Gene Expression Regulation, Enzymologic
MH  - Metalloendopeptidases/*genetics/*metabolism
MH  - Temperature
MH  - Vibrio vulnificus/*enzymology/*genetics
EDAT- 2004/10/27 09:00
MHDA- 2004/12/18 09:00
CRDT- 2004/10/27 09:00
PHST- 2004/06/18 00:00 [received]
PHST- 2004/08/25 00:00 [revised]
PHST- 2004/09/10 00:00 [accepted]
PHST- 2004/10/27 09:00 [pubmed]
PHST- 2004/12/18 09:00 [medline]
PHST- 2004/10/27 09:00 [entrez]
AID - S0378-1097(04)00686-X [pii]
AID - 10.1016/j.femsle.2004.09.023 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2004 Nov 1;240(1):55-9. doi: 10.1016/j.femsle.2004.09.023.