PMID- 15502216
OWN - NLM
STAT- MEDLINE
DCOM- 20050222
LR  - 20190902
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 291
DP  - 2005
TI  - The comet-FISH technique: a tool for detection of specific DNA damage and repair.
PG  - 107-19
AB  - The comet-FISH technique described in this protocol is a tool to detect genome 
      region-specific DNA damage and repair. It is a combination of two established 
      techniques, the comet assay (or single-cell gel electrophoresis, or the 
      single-cell gel test), to separate highly fragmented from moderately or 
      nonfragmented DNA and to measure it, and fluorescence in situ hybridization 
      (FISH), to specifically label DNA sequences of interest. Comet-FISH exists in two 
      versions, based on the neutral and the alkaline comet assays. A detailed 
      description of the comet assay is given in Chapter 9, so readers who are not 
      familiar with this technique can work directly with the protocol described here, 
      without referring to additional protocols reported elsewhere. The neutral version 
      of the comet assay detects double-strand breaks, while the alkaline version 
      detects both double- and single-strand breaks as well as abasic sites or sites of 
      incomplete repair. This chapter also details cell preparation and production of 
      the hybridization probes adapted to the comet-FISH technique. Finally, 
      microscopic analysis of comet-FISH results is described, and possible procedures 
      of quantification of the specific DNA damage are presented.
FAU - Rapp, Alexander
AU  - Rapp A
AD  - Department for Single Cell and Single Molecule Techniques, Institute for 
      Molecular Biotechnology Jena, Germany.
FAU - Hausmann, Michael
AU  - Hausmann M
FAU - Greulich, Karl Otto
AU  - Greulich KO
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Comet Assay/*methods
MH  - *DNA Damage
MH  - *DNA Repair
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 2004/10/27 09:00
MHDA- 2005/02/23 09:00
CRDT- 2004/10/27 09:00
PHST- 2004/10/27 09:00 [pubmed]
PHST- 2005/02/23 09:00 [medline]
PHST- 2004/10/27 09:00 [entrez]
AID - 1-59259-840-4:107 [pii]
AID - 10.1385/1-59259-840-4:107 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2005;291:107-19. doi: 10.1385/1-59259-840-4:107.