PMID- 15503807
OWN - NLM
STAT- MEDLINE
DCOM- 20050408
LR  - 20191109
IS  - 1567-2379 (Print)
IS  - 1567-2379 (Linking)
VI  - 35
IP  - 4
DP  - 2004 May
TI  - Localisation of the vacuolar proton pump (V-H+ -ATPase) and carbonic anhydrase II 
      in the human eccrine sweat gland.
PG  - 339-45
AB  - The localisation of the vacuolar proton pump (V-H+ -ATPase) and the enzyme 
      carbonic anhydrase II (CAII) was investigated in the human eccrine sweat gland 
      employing standard immunohistochemical techniques after antigen retrieval using 
      microwave heat treatment and high pressure. The high-pressure antigen retrieval 
      unmasked the presence of V-H+ -ATPase in the clear cells of the secretory coil, 
      with a distribution similar to that previously observed for CAII. However, the 
      dark cells were unreactive to both antibodies. In addition, heat and 
      high-pressure antigen retrieval demonstrated the presence of CAII in the apical 
      zone of luminal cells of the reabsorptive duct, a location not previously 
      reported. The localisation of V-H+ -ATPase and CAII in the secretory coil clear 
      cells suggests that the formation of HCO3- and H+ by carbonic anhydrase II and 
      the transport of H+ by V-H+ -ATPase may play an role in sweat fluid secretion. 
      Their presence at the apex of the duct cells indicates involvement in ductal ion 
      reabsorption.
FAU - Clunes, M T
AU  - Clunes MT
AD  - School of Biological and Biomedical Sciences, Glasgow Caledonian University, 
      Glasgow G4 0BA, UK.
FAU - Lindsay, S L
AU  - Lindsay SL
FAU - Roussa, E
AU  - Roussa E
FAU - Quinton, P M
AU  - Quinton PM
FAU - Bovell, D L
AU  - Bovell DL
LA  - eng
GR  - DK 51899/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
SB  - IM
MH  - Eccrine Glands/cytology/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Skin/anatomy & histology/*metabolism
MH  - Vacuolar Proton-Translocating ATPases/*metabolism
EDAT- 2004/10/27 09:00
MHDA- 2005/04/09 09:00
CRDT- 2004/10/27 09:00
PHST- 2004/10/27 09:00 [pubmed]
PHST- 2005/04/09 09:00 [medline]
PHST- 2004/10/27 09:00 [entrez]
AID - 10.1023/b:hijo.0000039837.45595.a6 [doi]
PST - ppublish
SO  - J Mol Histol. 2004 May;35(4):339-45. doi: 10.1023/b:hijo.0000039837.45595.a6.