PMID- 15503823
OWN - NLM
STAT- MEDLINE
DCOM- 20050216
LR  - 20171206
IS  - 0393-6155 (Print)
IS  - 0393-6155 (Linking)
VI  - 19
IP  - 3
DP  - 2004 Jul-Sep
TI  - Antibody response after vaccination with antigen-pulsed dendritic cells.
PG  - 213-20
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells of the immune 
      system capable of initiating immune responses to antigens. It is also well 
      documented that cancer patients often experience anergy against tumor antigens. 
      In this study we selected the best protocol for inducing the production of 
      antibodies against the HER2 oncoprotein using DCs to overcome anergy. Murine DCs 
      were pulsed in vitro, using different protocols, with recombinant HER2 fused to a 
      human Fc (in order to improve DC antigen uptake) and were used to vaccinate mice. 
      The obtained results indicate that antigen-pulsed DCs can induce an antibody 
      response and that adding CpG after antigen pulsing greatly increases anti-HER2 
      antibody production.
FAU - Battaini, F
AU  - Battaini F
AD  - Molecular Targeting Unit, Istituto Nazionale Tumori, University of Milan, 
      Milan--Italy.
FAU - Besusso, D
AU  - Besusso D
FAU - Sfondrini, L
AU  - Sfondrini L
FAU - Rossini, A
AU  - Rossini A
FAU - Morelli, D
AU  - Morelli D
FAU - Tagliabue, E
AU  - Tagliabue E
FAU - Menard, S
AU  - Menard S
FAU - Balsari, A
AU  - Balsari A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Biol Markers
JT  - The International journal of biological markers
JID - 8712411
RN  - 0 (Antibodies, Neoplasm)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cancer Vaccines)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Receptors, Fc)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Animals
MH  - Antibodies, Neoplasm/classification/*immunology
MH  - Antibody Formation
MH  - Antigens, Neoplasm/*immunology
MH  - B-Lymphocytes/immunology
MH  - Breast Neoplasms/immunology
MH  - Cancer Vaccines/*immunology
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - *Dendritic Cells/cytology/drug effects/*immunology/metabolism
MH  - Female
MH  - Humans
MH  - Immunoglobulin Class Switching/immunology
MH  - Immunoglobulin Isotypes/biosynthesis/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptor, ErbB-2/immunology
MH  - Receptors, Fc/immunology/metabolism
EDAT- 2004/10/27 09:00
MHDA- 2005/02/17 09:00
CRDT- 2004/10/27 09:00
PHST- 2004/10/27 09:00 [pubmed]
PHST- 2005/02/17 09:00 [medline]
PHST- 2004/10/27 09:00 [entrez]
PST - ppublish
SO  - Int J Biol Markers. 2004 Jul-Sep;19(3):213-20.