PMID- 15505075
OWN - NLM
STAT- MEDLINE
DCOM- 20041208
LR  - 20151119
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 45
IP  - 11
DP  - 2004 Nov
TI  - In vivo three-dimensional evaluation of leukocyte behavior in retinal 
      microcirculation of mice.
PG  - 4197-201
AB  - PURPOSE: To evaluate new physiologic and three-dimensional methods for monitoring 
      leukocyte behavior in mouse retina. METHODS: Endotoxin-induced uveitis (EIU) was 
      produced in mice by footpad injection of lipopolysaccharide (LPS). Leukocytes 
      were labeled with acridine orange (AO). Leukocyte rolling in the retinal 
      microcirculation was evaluated in vivo with AO digital fluorography. The number 
      of migrated leukocytes was counted in flatmounted retina. The behavior of 
      leukocyte migration was observed three-dimensionally at the time of peak 
      migration. After leukocytes were labeled with AO, the mice were perfused with 
      rhodamine-labeled concanavalin A lectin to stain the vascular endothelium. 
      Leukocyte migration into the retina was then monitored three-dimensionally with 
      confocal microscopy, and the velocity of the migration was measured. RESULTS: 
      Both leukocyte rolling and migration peaked at 48 hours after LPS injection. 
      Leukocytes were seen to extravasate from the deeper capillary layers and to 
      migrate toward the outer layer of the retina. The traveling velocity of 
      extravasated leukocytes in retinal tissue was 2.0 +/- 0.1 microm/h. CONCLUSIONS: 
      New methods have been demonstrated for the three-dimensional and quantitative 
      evaluation of leukocyte behavior in mouse retina.
FAU - Miyahara, Shinsuke
AU  - Miyahara S
AD  - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School 
      of Medicine, Kyoto, Japan.
FAU - Kiryu, Junichi
AU  - Kiryu J
FAU - Miyamoto, Kazuaki
AU  - Miyamoto K
FAU - Katsuta, Hideto
AU  - Katsuta H
FAU - Hirose, Fumitaka
AU  - Hirose F
FAU - Tamura, Hiroshi
AU  - Tamura H
FAU - Musashi, Kunihiro
AU  - Musashi K
FAU - Honda, Yoshihito
AU  - Honda Y
FAU - Yoshimura, Nagahisa
AU  - Yoshimura N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lipopolysaccharides)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Disease Models, Animal
MH  - Endothelium, Vascular/metabolism
MH  - Fluorescent Dyes
MH  - Fluorophotometry
MH  - Leukocyte Count
MH  - Leukocytes/*physiology
MH  - Lipopolysaccharides/toxicity
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microcirculation
MH  - Microscopy, Confocal
MH  - Retinal Vessels/*physiology
MH  - *Salmonella typhimurium
MH  - Uveitis/chemically induced/*physiopathology
EDAT- 2004/10/27 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/27 09:00
PHST- 2004/10/27 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/27 09:00 [entrez]
AID - 45/11/4197 [pii]
AID - 10.1167/iovs.04-0192 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2004 Nov;45(11):4197-201. doi: 10.1167/iovs.04-0192.