PMID- 15507300
OWN - NLM
STAT- MEDLINE
DCOM- 20041229
LR  - 20051116
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 102
IP  - 3
DP  - 2004 Dec 8
TI  - Apoptosis and porcine reproductive and respiratory syndrome virus.
PG  - 131-42
AB  - Despite numerous studies examining the possible induction of apoptosis in porcine 
      reproductive and respiratory syndrome virus (PRRSV)-infected cells, it remains 
      unclear if PRRSV infection results in direct apoptotic induction. There is clear 
      evidence that apoptotic cells are present in tissues from PRRSV-infected pigs. 
      However, many of these studies have failed to show that the apoptotic cells are 
      infected with PRRSV. This has led some investigators to propose that "bystander" 
      cells, not infected cells, become apoptotic during PRRSV infection by a yet 
      undetermined mechanism. Studies examining the induction of the apoptotic gene 
      expression response to PRRSV infection are needed to determine if PRRSV 
      replication triggers an apoptotic response. We have utilized microarray and 
      semi-quantitative reverse-transcription polymerase chain reaction (sqRT-PCR) to 
      evaluate apoptotic gene expression in PRRSV-infected MARC-145 cells. Twenty-six 
      apoptosis-related genes were examined during the first 24 h of infection and 
      found to be unaltered, indicating that apoptotic induction was not occurring in 
      PRRSV-infected cells. Additionally, using detection of free nucleosomal 
      complexes, we examined cells for both apoptotic and necrotic death resulting from 
      PRRSV infection at varying multiplicities of infection. This study indicates that 
      PRRSV-infected MARC-145 cells undergo necrosis at a much higher level than 
      apoptosis, and increases with virus levels used to infect the cells.
FAU - Miller, Laura C
AU  - Miller LC
AD  - Roman L. Hruska U.S. Meat Animal Research Center (MARC), ARS, USDA, State Spur 
      18D, P.O. Box 166, Clay Center, NE 68933-0166, USA.
FAU - Fox, James M
AU  - Fox JM
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
SB  - IM
MH  - Animals
MH  - *Apoptosis/genetics
MH  - Cell Line
MH  - Gene Expression Profiling/veterinary
MH  - Gene Expression Regulation
MH  - Necrosis/veterinary/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Swine/virology
MH  - Transcription, Genetic
RF  - 30
EDAT- 2004/10/28 09:00
MHDA- 2004/12/30 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2004/12/30 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - S0165-2427(04)00234-X [pii]
AID - 10.1016/j.vetimm.2004.09.004 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Dec 8;102(3):131-42. doi: 
      10.1016/j.vetimm.2004.09.004.