PMID- 15507305
OWN - NLM
STAT- MEDLINE
DCOM- 20041229
LR  - 20210119
IS  - 0165-2427 (Print)
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 102
IP  - 3
DP  - 2004 Dec 8
TI  - Differential host cell gene expression regulated by the porcine reproductive and 
      respiratory syndrome virus GP4 and GP5 glycoproteins.
PG  - 189-98
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) GP4 and GP5 
      proteins are two membrane-associated viral glycoproteins that have been shown to 
      induce neutralizing antibodies. In the present study, the host cell gene 
      expression profiles altered by the GP4 and GP5 proteins were investigated by the 
      use of DNA microarrays. Sublines of Marc-145 and HeLa cells were established by 
      stable transfection with open reading frame (ORF)4 and ORF5 of PRRSV, 
      respectively, and differential gene expressions were studied using microarray 
      chips embedded with 1718 human-expressed sequence tags. The genes for protein 
      degradation, protein synthesis and transport, and various other biochemical 
      pathways were identified. No genes involved in the apoptosis pathway appeared to 
      be regulated in GP5-expressing cells. The microarray data may provide insights 
      into the specific cellular responses to the GP4 and GP5 proteins during PRRSV 
      infection.
FAU - Lee, Changhee
AU  - Lee C
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph, Ont., N1G 2W1, Canada.
FAU - Bachand, Aimee
AU  - Bachand A
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
FAU - Yoo, Dongwan
AU  - Yoo D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*physiology
MH  - Glycoproteins/*physiology
MH  - Humans
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Viral Envelope Proteins/*physiology
MH  - Viral Structural Proteins/*physiology
PMC - PMC7112691
EDAT- 2004/10/28 09:00
MHDA- 2004/12/30 09:00
PMCR- 2004/10/18
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2004/12/30 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
PHST- 2004/10/18 00:00 [pmc-release]
AID - S0165-2427(04)00251-X [pii]
AID - 10.1016/j.vetimm.2004.09.020 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Dec 8;102(3):189-98. doi: 
      10.1016/j.vetimm.2004.09.020.