PMID- 15507308
OWN - NLM
STAT- MEDLINE
DCOM- 20041229
LR  - 20220317
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 102
IP  - 3
DP  - 2004 Dec 8
TI  - Pathogenic and humoral immune responses to porcine reproductive and respiratory 
      syndrome virus (PRRSV) are related to viral load in acute infection.
PG  - 233-47
AB  - Although much research has been performed on porcine reproductive and respiratory 
      syndrome virus (PRRSV), little quantitative information is available on the 
      relationships between virulence and in vivo virus replication, among isolates 
      recovered at different times in the history of PRRS, or the relative levels of 
      virulence associated with individual virus isolates. In this study, the in vivo 
      growth properties of virulent field isolates and attenuated PRRSV isolates were 
      compared. The results show that virulent PRRSV isolates exhibit longer and more 
      elevated levels of viremia, induce faster and more intense humoral immune 
      responses, negatively affect body weight gain, induce higher death rates, and 
      cause more severe clinical signs in a respiratory disease model. We found that 
      the more virulent field isolates grew to significantly higher levels in pigs than 
      did cell-culture adapted isolates. We concluded that the pathogenic consequences 
      and immunological responses of pigs to PRRSV are directly related to viral load 
      in acute infection as reflected in viral titers in blood.
FAU - Johnson, Wesley
AU  - Johnson W
AD  - Boehringer Ingelheim Vetmedica, Inc., 2501 North Loop Drive, Suite 1000, Ames, IA 
      50010-8612, USA. wjohnso2@bi-vetmedica.com
FAU - Roof, Michael
AU  - Roof M
FAU - Vaughn, Eric
AU  - Vaughn E
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
FAU - Johnson, Craig R
AU  - Johnson CR
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/pathology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*immunology/pathogenicity
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine/*immunology/virology
MH  - Time Factors
MH  - Viral Load/*veterinary
MH  - Viremia/immunology/veterinary
MH  - Virulence/immunology
MH  - Virus Replication/immunology
EDAT- 2004/10/28 09:00
MHDA- 2004/12/30 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2004/12/30 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - S0165-2427(04)00258-2 [pii]
AID - 10.1016/j.vetimm.2004.09.010 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Dec 8;102(3):233-47. doi: 
      10.1016/j.vetimm.2004.09.010.