PMID- 15507312
OWN - NLM
STAT- MEDLINE
DCOM- 20041229
LR  - 20081121
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 102
IP  - 3
DP  - 2004 Dec 8
TI  - Immune responses of swine following DNA immunization with plasmids encoding 
      porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine 
      IL-2 and IFNgamma.
PG  - 291-8
AB  - In the present study, five eukaryotic double-gene expression plasmids containing 
      porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 and ORF7 genes 
      combined with cDNAs encoding porcine IFNgamma and IL-2 were constructed for 
      evaluation as PRRSV vaccine candidates. After immunization and viral challenge, 
      two of three pigs immunized with pIRESorf5/IFNgamma, one of three pigs immunized 
      with pIRESorf5/IL-2 and one of three pigs immunized with pIRESorf7/IL-2 were 
      protected from lung lesions that were present in other vaccinated and control 
      animals. Virus replication was reduced but not completely prevented in organs of 
      the DNA-vaccinated animals as compared to controls. Therefore, the porcine 
      cytokines IFNgamma and IL-2, delivered in combination with ORF5 or ORF7, may 
      improve the immune efficacy of DNA vaccines against PRRSV.
FAU - Xue, Qiang
AU  - Xue Q
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, CAAS, 427 Maduan Street, Harbin 150001, PR China.
FAU - Zhao, Yong-Gang
AU  - Zhao YG
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - Qiu, Hua-Ji
AU  - Qiu HJ
FAU - Wang, Yun-Feng
AU  - Wang YF
FAU - Wu, Dong-Lai
AU  - Wu DL
FAU - Tian, Zhi-Jun
AU  - Tian ZJ
FAU - Tong, Guang-Zhi
AU  - Tong GZ
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antiviral Agents)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-2)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Vaccines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antiviral Agents/immunology
MH  - DNA, Complementary/immunology
MH  - Interferon-gamma/*immunology
MH  - Interleukin-2/*immunology
MH  - Open Reading Frames/*immunology
MH  - Plasmids/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine/*immunology/*virology
MH  - Vaccines, DNA/immunology
MH  - Viral Vaccines/immunology
EDAT- 2004/10/28 09:00
MHDA- 2004/12/30 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2004/12/30 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - S0165-2427(04)00262-4 [pii]
AID - 10.1016/j.vetimm.2004.09.021 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Dec 8;102(3):291-8. doi: 
      10.1016/j.vetimm.2004.09.021.