PMID- 15507314
OWN - NLM
STAT- MEDLINE
DCOM- 20041229
LR  - 20081121
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 102
IP  - 3
DP  - 2004 Dec 8
TI  - Beta-glucan enhancement of T cell IFNgamma response in swine.
PG  - 315-20
AB  - Beta-glucan has been shown to enhance anti-tumor and anti-infection functions in 
      animals. Pigs at 4 months of age were infected with porcine reproductive and 
      respiratory syndrome virus (PRRSV), and peripheral blood monocytes (PBMC) were 
      isolated for the detection of interferon gamma (IFNgamma)-producing cells. We 
      found that soluble high molecular weight beta-glucan could increase 
      IFNgamma-producing cell frequency in a dose-dependent manner in the enzyme-linked 
      immunospot assay (ELISPOT) in the absence of antigenic restimulation. A 
      concentration as low as 1.6 microg/ml gave a significant increase and a similarly 
      high enhancement was achieved at concentrations from 3.2 to 100 microg/ml. In 
      PRRSV-specific IFNgamma ELISPOT, soluble beta-glucan elicited increased 
      PRRSV-specific responses at concentrations from 3.2 to 50 microg/ml, but not at 
      100 microg/ml, whereas insoluble beta-glucan had no effect. Soluble beta-glucan 
      augmented the porcine cellular immune response in an antigen-independent fashion, 
      whereas insoluble beta-glucan had no activity. This finding suggests that soluble 
      beta-glucan may enhance innate antiviral immunity against PRRSV.
FAU - Xiao, Zhengguo
AU  - Xiao Z
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 
      Commonwealth Avenue, St. Paul, MN 55108, USA.
FAU - Trincado, Carlos A
AU  - Trincado CA
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Immunologic Factors)
RN  - 0 (beta-Glucans)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Female
MH  - Immunologic Factors/*pharmacology
MH  - Interferon-gamma/*biosynthesis
MH  - Porcine Reproductive and Respiratory Syndrome/immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine/*immunology
MH  - T-Lymphocytes/*immunology
MH  - beta-Glucans/*pharmacology
EDAT- 2004/10/28 09:00
MHDA- 2004/12/30 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2004/12/30 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - S0165-2427(04)00264-8 [pii]
AID - 10.1016/j.vetimm.2004.09.013 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Dec 8;102(3):315-20. doi: 
      10.1016/j.vetimm.2004.09.013.