PMID- 15507315
OWN - NLM
STAT- MEDLINE
DCOM- 20041229
LR  - 20061115
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 102
IP  - 3
DP  - 2004 Dec 8
TI  - Humoral immune response induced by oral administration of S. typhimurium 
      containing a DNA vaccine against porcine reproductive and respiratory syndrome 
      virus.
PG  - 321-8
AB  - The ORFs-encoded major envelope glycoprotein (GP5) of porcine reproductive and 
      respiratory syndrome virus (PRRSV) is one of the major structural proteins of 
      this virus. In this report, we described the induction of a PRRSV GP5-specific 
      immune response by oral vaccination of mice with eukaryotic expression vectors 
      containing the GP5 gene of PRRSV (pcDNA3-GP5), delived by attenuated Salmonella 
      typhimurium aroA. It demonstrated that oral administration of the transformants 
      resulted in expression of the GP5 transcript in the intestinal epithelium. The 
      level of serum neutralization antibodies to PRRSV was not significantly different 
      between the mice immunized with the transformants and the naked plasmid DNA. But 
      the neutralizing antibody titres in sera of the mice immunized with 
      SL7207/pcDNA3-GP5' and pcDNA3-GP5' (resides 2-25 deletion mutant of GP5) were 
      significantly lower than those immunized with the complete GP5 gene. These 
      results show that oral inoculation of the transformants can induce humoral immune 
      response to PRRSV. The signal peptide of the GP5 protein of PRRSV is associated 
      with the neutralizing epitope of the protein. The attenuated S. typhimurium may 
      be used as a delivery system for oral DNA vaccines containing PRRSV GP5 
      glycoprotein.
FAU - Jiang, Ping
AU  - Jiang P
AD  - Key Laboratory of Animal Diseases Diagnostic and Immunology, College of 
      Veterinary Medicine, Nanjing Agriculture University, Ministry of Agriculture, 
      Nanjing 210095, China. jiangp@njau.edu.cn
FAU - Jiang, Wenming
AU  - Jiang W
FAU - Li, Yufeng
AU  - Li Y
FAU - Wu, Shengxie
AU  - Wu S
FAU - Xu, Jiarong
AU  - Xu J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Female
MH  - Gene Expression
MH  - Injections, Intradermal
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Open Reading Frames/immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Salmonella typhimurium
MH  - Vaccines, DNA/*administration & dosage/*immunology
MH  - Viral Envelope Proteins/immunology
MH  - Viral Vaccines/*administration & dosage/*immunology
EDAT- 2004/10/28 09:00
MHDA- 2004/12/30 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2004/12/30 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - S0165-2427(04)00265-X [pii]
AID - 10.1016/j.vetimm.2004.09.014 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Dec 8;102(3):321-8. doi: 
      10.1016/j.vetimm.2004.09.014.