PMID- 15507316
OWN - NLM
STAT- MEDLINE
DCOM- 20041229
LR  - 20071114
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 102
IP  - 3
DP  - 2004 Dec 8
TI  - Oral immunization induces local and distant mucosal immunity in swine.
PG  - 329-38
AB  - Transmission of porcine reproductive and respiratory syndrome virus (PRRSV) in 
      semen and reproductive disease in pregnant swine might be reduced by vaccines 
      that induce mucosal immunity in the reproductive tract. Cholera toxin (CT), when 
      delivered orally, is a potent mucosal adjuvant and immunogen in swine. To 
      determine if oral immunization additionally elicits immunity at distant mucosal 
      surfaces, we examined antibody responses to CT-B subunit in the reproductive 
      tract and oral cavity. Orally administered CT induced distant mucosal immunity, 
      as measured by antibodies to CT-B subunit in saliva and vaginal secretions. 
      Presentation of PRRSV nucleocapsid as a genetic fusion with CT resulted in local 
      mucosal antibody production, but no response was observed in vaginal secretions. 
      The results demonstrate the feasibility of using orally administered CT for the 
      induction of immunity to reproductive pathogens in swine. However, effective 
      induction of PRRSV-specific immune responses in the reproductive tract requires a 
      better understanding of the mechanisms of antigenicity and adjuvanticity at 
      distant mucosal sites.
FAU - Hyland, Kendra
AU  - Hyland K
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 
      Commonwealth Avenue, St. Paul, MN 55108, USA.
FAU - Foss, Dennis L
AU  - Foss DL
FAU - Johnson, Craig R
AU  - Johnson CR
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
LA  - eng
GR  - 5 T32 DA-07097-22/DA/NIDA NIH HHS/United States
GR  - K08AI01396/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Viral)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vaccines, Conjugate)
RN  - 0 (Viral Vaccines)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Viral/biosynthesis/blood
MH  - Cholera Toxin/immunology
MH  - Female
MH  - Immunity, Mucosal/*immunology
MH  - Intestinal Mucosa/immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Recombinant Fusion Proteins/immunology
MH  - Swine/*immunology
MH  - Vaccines, Conjugate/immunology
MH  - Viral Vaccines/*administration & dosage/immunology
EDAT- 2004/10/28 09:00
MHDA- 2004/12/30 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2004/12/30 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - S0165-2427(04)00266-1 [pii]
AID - 10.1016/j.vetimm.2004.09.015 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Dec 8;102(3):329-38. doi: 
      10.1016/j.vetimm.2004.09.015.