PMID- 15508178
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20191210
IS  - 1613-4125 (Print)
IS  - 1613-4125 (Linking)
VI  - 48
IP  - 6
DP  - 2004 Nov
TI  - Evaluation of the potential allergenicity of the enzyme microbial 
      transglutaminase using the 2001 FAO/WHO Decision Tree.
PG  - 434-40
AB  - All novel proteins must be assessed for their potential allergenicity before they 
      are introduced into the food market. One method to achieve this is the 2001 
      FAO/WHO Decision Tree recommended for evaluation of proteins from genetically 
      modified organisms (GMOs). It was the aim of this study to investigate the 
      allergenicity of microbial transglutaminase (m-TG) from Streptoverticillium 
      mobaraense. Amino acid sequence similarity to known allergens, pepsin resistance, 
      and detection of protein binding to specific serum immunoglobulin E (IgE) (RAST) 
      have been evaluated as recommended by the decision tree. Allergenicity in the 
      source material was thought unlikely, since no IgE-mediated allergy to any 
      bacteria has been reported. m-TG is fully degraded after 5 min of pepsin 
      treatment. A database search showed that the enzyme has no homology with known 
      allergens, down to a match of six contiguous amino acids, which meets the 
      requirements of the decision tree. However, there is a match at the five 
      contiguous amino acid level to the major codfish allergen Gad c1. The potential 
      cross reactivity between m-TG and Gad c1 was investigated in RAST using sera from 
      25 documented cod-allergic patients and an extract of raw codfish. No binding 
      between patient IgE and m-TG was observed. It can be concluded that no safety 
      concerns with regard to the allergenic potential of m-TG were identified.
FAU - Pedersen, Mona H
AU  - Pedersen MH
AD  - Laboratory of Medical Allergology, Allergy Clinic, National University Hospital, 
      Copenhagen, Denmark.
FAU - Hansen, Tine K
AU  - Hansen TK
FAU - Sten, Eva
AU  - Sten E
FAU - Seguro, Katsuya
AU  - Seguro K
FAU - Ohtsuka, Tomoko
AU  - Ohtsuka T
FAU - Morita, Akiko
AU  - Morita A
FAU - Bindslev-Jensen, Carsten
AU  - Bindslev-Jensen C
FAU - Poulsen, Lars K
AU  - Poulsen LK
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Immune Sera)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.3.2.13 (Transglutaminases)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Animals
MH  - *Consumer Product Safety
MH  - Cross Reactions
MH  - *Decision Trees
MH  - Food Hypersensitivity/*prevention & control
MH  - Gadus morhua/immunology
MH  - Humans
MH  - Immune Sera/immunology
MH  - Immunoglobulin E/biosynthesis/immunology
MH  - Organisms, Genetically Modified
MH  - Pepsin A/metabolism
MH  - Risk Assessment
MH  - Sequence Homology, Amino Acid
MH  - Streptomyces/enzymology
MH  - Transglutaminases/chemistry/genetics/*immunology/metabolism
MH  - Trypsin/metabolism
EDAT- 2004/10/28 09:00
MHDA- 2005/04/22 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - 10.1002/mnfr.200400014 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2004 Nov;48(6):434-40. doi: 10.1002/mnfr.200400014.