PMID- 15508180
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20151119
IS  - 1613-4125 (Print)
IS  - 1613-4125 (Linking)
VI  - 48
IP  - 6
DP  - 2004 Nov
TI  - Development of an epitope-specific analytical tool for the major peanut allergen 
      Ara h 2 using a high-density multiple-antigenic peptide strategy.
PG  - 449-58
AB  - Using the major peanut allergen Ara h 2 as an example, an analytical tool 
      enabling the determination of immunoglobulin E (IgE)-epitopes in processed food 
      allergens was developed. We synthesized a multiple-antigenic peptide (MAP) of the 
      IgE-reactive linear epitope 3 (amino acid positions 27-36) of Ara h 2 and raised 
      a monospecific antiserum against this epitope to obtain a positive control for 
      future epitope resolved diagnostics. First, a MAP of epitope 3, having a 
      molecular mass of 7770 Da, was synthesized, purified, and its structure confirmed 
      by liquid chromatography-mass spectrometry (electrospray ionization) 
      (LC-MS(ESI)), matrix assisted laser desorption/ionization-time of flight 
      (MALDI-TOF), and Edman sequencing. The MAP was then used to raise high titer 
      antibodies in rabbits using the adjuvant Titermax and to characterize the 
      specificity of IgE from allergenic patients sensitized to Ara h 2. The antiserum 
      exclusively detects Ara h 2 in crude peanut extract with a titer of 10(7) by 
      Western blot and reacts specifically with epitope 3 shown by epitope mapping for 
      a library of solid-phase-bound synthetic 15-mer peptides covering the entire 
      sequence of Ara h 2. Such IgE-reactive epitopes are of high analytical relevance 
      as they could constitute the basis for epitope-specific detection systems for use 
      in quality control in the food industry or for forensic purposes in cases of 
      fatal reactions to otherwise undetected peanut proteins.
FAU - Gruber, Patrick
AU  - Gruber P
AD  - Deutsche Forschungsanstalt fur Lebensmittelchemie, Garching, Germany.
FAU - Suhr, Martin
AU  - Suhr M
FAU - Frey, Andreas
AU  - Frey A
FAU - Becker, Wolf-Meinhard
AU  - Becker WM
FAU - Hofmann, Thomas
AU  - Hofmann T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (2S Albumins, Plant)
RN  - 0 (Allergens)
RN  - 0 (Antigens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Ara h II protein, Arachis hypogaea)
RN  - 0 (Epitopes)
RN  - 0 (Glycoproteins)
RN  - 0 (Immune Sera)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - 2S Albumins, Plant
MH  - Allergens/chemistry/genetics/*immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens/immunology
MH  - Antigens, Plant
MH  - *Arachis
MH  - Blotting, Western
MH  - Chromatography, High Pressure Liquid
MH  - *Epitope Mapping
MH  - Epitopes/chemistry/immunology/isolation & purification
MH  - Gas Chromatography-Mass Spectrometry
MH  - Glycoproteins/chemistry/genetics/*immunology
MH  - Humans
MH  - Immune Sera/immunology
MH  - Immunoglobulin E/immunology
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Peanut Hypersensitivity/*immunology
MH  - Plant Proteins
MH  - Rabbits
MH  - Sequence Analysis, Protein
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2004/10/28 09:00
MHDA- 2005/04/22 09:00
CRDT- 2004/10/28 09:00
PHST- 2004/10/28 09:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2004/10/28 09:00 [entrez]
AID - 10.1002/mnfr.200400005 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2004 Nov;48(6):449-58. doi: 10.1002/mnfr.200400005.