PMID- 15508750
OWN - NLM
STAT- MEDLINE
DCOM- 20050408
LR  - 20191026
IS  - 1343-4934 (Print)
IS  - 1343-4934 (Linking)
VI  - 46
IP  - 3
DP  - 2004 Sep
TI  - Telomeres on chromosome 21 and aging in lymphocytes and gingival fibroblasts from 
      individuals with Down syndrome.
PG  - 171-7
AB  - Progressive chromosome 21 loss in individuals with trisomy 21 or Down syndrome 
      (DS) is supposedly related to their premature senescence. In addition, the 
      telomere hypothesis of cellular aging involving telomere shortening in normal and 
      accelerated aging in vivo and in vitro is well documented. This study 
      investigated the integrity of two chromosome 21 regions (the 21q telomere and the 
      21q22.13-q22.2 region) and their relationship with aging by means of fluorescence 
      in situ hybridization (FISH) in lymphocytes and gingival fibroblasts cells. The 
      use of tissues from different germ layers allows detection of mosaicism. 
      Chromosome variations in tissue from the neuroectoderm layer could explain the 
      variable phenotype of DS. This approach is original in the literature. Lymphocyte 
      and gingival fibroblast nuclei from 18 affected individuals aged 5-54 years were 
      analyzed. Although not significant (P = 0.06), analysis from 11 tissue-matched 
      individuals as well as the comparison between lymphocytes and fibroblasts from 
      different subjects (P = 0.05) suggested that lymphocyte cells are more likely to 
      miss 21q telomere signals. Hence, gingival fibroblasts are probably capable of 
      more efficient cell repair, and the occurrence of mosaicism is more related to 
      cell proliferation than to germ layer origin. Investigation of the 21q22.13-q22.2 
      region from six tissue-matched individuals and from different DS patients 
      revealed no significant differences between the tissues.
FAU - de Arruda Cardoso Smith, Marilia
AU  - de Arruda Cardoso Smith M
AD  - Disciplina de Genetica, Departamento de Morfologia, Universidade Federal de Sao 
      Paulo/Escola Paulista de Medicina, UNIFESP/EPM, Sao Paulo, Brazil. 
      macsmith.morf@epm.br
FAU - Borsatto-Galera, Bianca
AU  - Borsatto-Galera B
FAU - Feller, Roger Israel
AU  - Feller RI
FAU - Goncalves, Alaide
AU  - Goncalves A
FAU - Oyama, Rosa Sayoto Kawasaki
AU  - Oyama RS
FAU - Segato, Rosemeire
AU  - Segato R
FAU - Chen, Elizabeth
AU  - Chen E
FAU - Carvalheira, Gianna Maria Griz
AU  - Carvalheira GM
FAU - Filho, Antonio Santos Clemente
AU  - Filho AS
FAU - Burbano, Rommel Rodriguez
AU  - Burbano RR
FAU - Payao, Spencer Luiz Marques
AU  - Payao SL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Oral Sci
JT  - Journal of oral science
JID - 9808942
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cellular Senescence/*genetics
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Down Syndrome/*genetics
MH  - Fibroblasts/*diagnostic imaging
MH  - Humans
MH  - Linear Models
MH  - Lymphocytes/*ultrastructure
MH  - Middle Aged
MH  - Statistics, Nonparametric
MH  - Telomere/*genetics
MH  - Ultrasonography
EDAT- 2004/10/29 09:00
MHDA- 2005/04/09 09:00
CRDT- 2004/10/29 09:00
PHST- 2004/10/29 09:00 [pubmed]
PHST- 2005/04/09 09:00 [medline]
PHST- 2004/10/29 09:00 [entrez]
AID - 10.2334/josnusd.46.171 [doi]
PST - ppublish
SO  - J Oral Sci. 2004 Sep;46(3):171-7. doi: 10.2334/josnusd.46.171.