PMID- 15509322
OWN - NLM
STAT- MEDLINE
DCOM- 20050628
LR  - 20190513
IS  - 1524-4040 (Electronic)
IS  - 0148-396X (Linking)
VI  - 55
IP  - 5
DP  - 2004 Nov
TI  - Direct detection of bacterial pathogens in brain abscesses by polymerase chain 
      reaction amplification and sequencing of partial 16S ribosomal deoxyribonucleic 
      acid fragments.
PG  - 1154-62
AB  - OBJECTIVE: To evaluate the feasibility of detecting bacterial pathogens directly 
      from the clinical brain abscess specimens by polymerase chain reaction (PCR) 
      amplification and sequencing of bacterial 16S ribosomal deoxyribonucleic acid 
      (rDNA). METHODS: A total of 14 specimens were tested by both culture and PCR 
      amplification, targeting the full-length or a partial region of 16S rDNA. 16S 
      rDNA is known to be conserved in bacteria. Sequencing of partial-length and 
      full-length 16S rDNA was performed. The sequence data were compared with known 
      sequences of 16S rDNA in the National Center for Biotechnology Information 
      GenBank by using the Basic Local Alignment Search Tool (BLAST) algorithm. The 
      species with the best match of similarity were regarded as the pathogenic species 
      in the samples. We also developed a Streptococcus-specific multiplex PCR analysis 
      for identifying members of the Streptococcus species, the most common pathogen of 
      brain abscesses. RESULTS: The 10 culture-positive specimens were all PCR-positive 
      for partial 16S rDNA, but only seven were positive for full-length 16S rDNA 
      amplification. Bacterial DNA was not detected in the remaining four specimens 
      with a negative culture. Species identification by phenotypes from culture was in 
      agreement with that by sequencing results of partial-length (or full-length) 16S 
      rDNA. The Streptococcus-specific PCR analysis could detect Streptococcus species 
      correctly in one step. CONCLUSION: Bacterial 16S rDNA sequences provide reliable 
      clues to the identification of unknown pathogens. PCR analysis of 16S rDNA and 
      sequencing may identify pathogens to the species level directly from brain 
      abscesses. This approach is rapid and is useful especially in the identification 
      of slow-growing and fastidious organisms.
FAU - Tsai, Jui-Chang
AU  - Tsai JC
AD  - Division of Neurosurgery, Department of Surgery, National Taiwan University 
      Hospital and Center for Optoelectronic Biomedicine, National Taiwan University 
      College of Medicine, Taipei, Taiwan. jctsai@ha.mc.ntu.edu.tw
FAU - Teng, Lee-Jene
AU  - Teng LJ
FAU - Hsueh, Po-Ren
AU  - Hsueh PR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurosurgery
JT  - Neurosurgery
JID - 7802914
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
RPI - Neurosurgery. 2008 Feb;62 Suppl 2:547-55. PMID: 18596449
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain Abscess/*microbiology
MH  - DNA, Bacterial/genetics
MH  - Enterobacter cloacae/genetics/growth & development/isolation & purification
MH  - Female
MH  - Fusobacterium/genetics/growth & development/*isolation & purification
MH  - Fusobacterium Infections/*diagnosis
MH  - Gram-Negative Bacteria/genetics/*isolation & purification
MH  - Gram-Negative Bacterial Infections/*diagnosis
MH  - Haemophilus/genetics/growth & development/isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Species Specificity
MH  - Staphylococcaceae/genetics/growth & development/isolation & purification
MH  - Staphylococcus aureus/genetics/growth & development/isolation & purification
MH  - Staphylococcus epidermidis/genetics/growth & development/isolation & purification
MH  - Streptococcus/genetics/growth & development/isolation & purification
EDAT- 2004/10/29 09:00
MHDA- 2005/06/29 09:00
CRDT- 2004/10/29 09:00
PHST- 2003/09/23 00:00 [received]
PHST- 2004/02/17 00:00 [accepted]
PHST- 2004/10/29 09:00 [pubmed]
PHST- 2005/06/29 09:00 [medline]
PHST- 2004/10/29 09:00 [entrez]
AID - 10.1227/01.neu.0000140842.37422.ee [doi]
PST - ppublish
SO  - Neurosurgery. 2004 Nov;55(5):1154-62. doi: 10.1227/01.neu.0000140842.37422.ee.