PMID- 15509364
OWN - NLM
STAT- MEDLINE
DCOM- 20050303
LR  - 20151119
IS  - 0022-2151 (Print)
IS  - 0022-2151 (Linking)
VI  - 118
IP  - 9
DP  - 2004 Sep
TI  - Studies of the cell cycle regulatory proteins P16, cyclin D1 and retinoblastoma 
      protein in laryngeal carcinoma tissue.
PG  - 676-80
AB  - Defects in the system controlling the cell cycle can lead to an increased 
      proliferation of cancer cells. The aim of this study was to analyse the 
      immunohistochemical expression of chosen cell cycle proteins (P16, cyclin D1 and 
      retinoblastoma protein) and their connection with the clinical course of the 
      disease in laryngeal squamous cell cancer (LSCC). Cancer tissue sections obtained 
      from 58 patients after total laryngectomy served to determine the level of the 
      proteins' expression using immunohistochemical staining and commercial 
      antibodies. A decreased level of P16 expression in 47 per cent, of retinoblastoma 
      protein in 12 per cent and strong cyclin D1 expression in 48 per cent of cases 
      was revealed. Our results show significant correlation between decreased P16 
      expression and increased tumour dedifferentiation. Overexpression of cyclin D1 
      was statistically more common in locally advanced tumours (T(3) -T(4)). Low 
      expression of retinoblastoma protein was significantly correlated with both 
      positive P16 immunostaining and with strong cyclin D1 expression. Our study 
      confirms that dysfunction of cell cycle regulation is a common event and may play 
      a significant role in the development of squamous cell carcinoma of the larynx.
FAU - Krecicki, Tomasz
AU  - Krecicki T
AD  - Department of Otolaryngology, Wroclaw Medical University, Poland. 
      krecicki@orl.am.wroc.pl
FAU - Smigiel, Robert
AU  - Smigiel R
FAU - Fraczek, Marcin
AU  - Fraczek M
FAU - Kowalczyk, Marlena
AU  - Kowalczyk M
FAU - Sasiadek, Maria M
AU  - Sasiadek MM
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Laryngol Otol
JT  - The Journal of laryngology and otology
JID - 8706896
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*metabolism
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Cycle Proteins/*metabolism
MH  - Cell Differentiation
MH  - Cyclin D1/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p16/metabolism
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*metabolism
MH  - Prognosis
MH  - Retinoblastoma Protein/metabolism
EDAT- 2004/10/29 09:00
MHDA- 2005/03/04 09:00
CRDT- 2004/10/29 09:00
PHST- 2004/10/29 09:00 [pubmed]
PHST- 2005/03/04 09:00 [medline]
PHST- 2004/10/29 09:00 [entrez]
AID - 10.1258/0022215042244769 [doi]
PST - ppublish
SO  - J Laryngol Otol. 2004 Sep;118(9):676-80. doi: 10.1258/0022215042244769.