PMID- 15510206
OWN - NLM
STAT- MEDLINE
DCOM- 20050401
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 19
IP  - 1
DP  - 2005 Jan
TI  - Development of a D-FISH method to detect DEK/CAN fusion resulting from 
      t(6;9)(p23;q34) in patients with acute myelogenous leukemia.
PG  - 126-31
AB  - The t(6;9)(p23;q34)-DEK/CAN fusion occurs with an incidence of 1-5% in adult 
      patients with acute myelogenous leukemia (AML) and tends to have an unfavorable 
      prognosis at diagnosis. Due to the subtle appearance of this chromosome 
      rearrangement, both initial detection and minimal residual disease (MRD) tracking 
      by conventional karyotyping can be difficult. Unfortunately, no commercial or 
      previously published fluorescence in situ hybridization (FISH) strategies exist 
      for this recurrent anomaly. We have developed a highly sensitive assay using 
      dual-color, double-fusion FISH (D-FISH), which can be used both for initial 
      detection and MRD monitoring. We analyzed archived bone marrow samples from 15 
      patients with a previously identified t(6;9)(p23;q34) and 10 corresponding 
      post-treatment samples. The results demonstrate that our D-FISH method 
      effectively identified all abnormal samples, including a low-level MRD sample 
      that was considered to be normal by conventional cytogenetic analysis. Normal 
      value ranges were established from 30 negative controls to be < 0.6% when 500 
      interphase nuclei were analyzed. The development of this sensitive D-FISH 
      strategy for the detection of the t(6;9)(p23;q34) adds to the AML FISH testing 
      repertoire, and is effective in the detection of low-level disease in 
      post-treatment samples in these patients.
FAU - Shearer, B M
AU  - Shearer BM
AD  - Department of Laboratory Medicine and Pathology, Division of Laboratory Genetics, 
      Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
FAU - Knudson, R A
AU  - Knudson RA
FAU - Flynn, H C
AU  - Flynn HC
FAU - Ketterling, R P
AU  - Ketterling RP
LA  - eng
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DEK-CAN fusion protein, recombinant)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - *Chromosomes, Human, Pair 6
MH  - *Chromosomes, Human, Pair 9
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics/pathology
MH  - Neoplasm, Residual
MH  - Oncogene Proteins/*genetics
MH  - Oncogene Proteins, Fusion
MH  - Recombinant Fusion Proteins/*genetics
MH  - *Translocation, Genetic
EDAT- 2004/10/29 09:00
MHDA- 2005/04/02 09:00
CRDT- 2004/10/29 09:00
PHST- 2004/10/29 09:00 [pubmed]
PHST- 2005/04/02 09:00 [medline]
PHST- 2004/10/29 09:00 [entrez]
AID - 2403557 [pii]
AID - 10.1038/sj.leu.2403557 [doi]
PST - ppublish
SO  - Leukemia. 2005 Jan;19(1):126-31. doi: 10.1038/sj.leu.2403557.