PMID- 15512948
OWN - NLM
STAT- MEDLINE
DCOM- 20041206
LR  - 20061115
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 28
IP  - 6
DP  - 2004 Jun
TI  - HLA expression in choroidal melanomas: correlation with clinicopathological 
      features.
PG  - 409-16
AB  - PURPOSE: In this retrospective study, we analyzed the relevance of human 
      leukocyte antigen (HLA) expression to clinical behavior of uveal melanoma and 
      correlated it with conventional light microscopic parameters. METHODS: HLA class 
      I antigen and Beta 2 microglobulin expression were analyzed in 45 primary 
      choroidal melanoma lesions by immunoperoxidase staining with monoclonal 
      antibodies to HLA class I antigen and beta 2 microglobulin and correlated with 
      the known clinicopathological parameters. Immunoanalysis was done by a semi 
      quantitative method. The tumors were divided into 3 groups. Group A: Tumors with 
      no extrascleral extension/no liver metastases, group B: tumors with only 
      extrascleral extension and group C: tumors with liver metastases. For statistical 
      analysis we analyzed the negative, weak (heterogeneous) and the positive 
      expression of HLA and beta 2 microglobulin with known clinicopathological 
      parameters. RESULTS: In-group A (n = 35) tumors with no extrascleral extension 
      and no liver metastases HLA class I antigen and beta 2 microglobulin are negative 
      (absent staining) in 30 choroidal melanomas. In-group B (n = 4) they are weak 
      (heterogeneous) in 3 tumors. In-group C (n = 6) all the 6 tumors have a positive 
      (bright staining) immunoexpression. No statistical significance was obtained when 
      HLA and beta 2 microglobulin immunoreactivity was compared against largest tumor 
      diameter (LTD), tumor infiltrating lymphocytes (TIL), mitosis and nuclear grade. 
      The difference of HLA class I and beta 2-microglobulin imunoreactivity among the 
      various cell types spindle, mixed and epithelioid was statistically significant 
      (p = 0.003), (p = 0.004). The difference in immunoreactivity between tumors with 
      no liver metastases and tumors with liver metastases was statistically 
      significant (p < 0.001). CONCLUSIONS: HLA class I antigen and beta 2 
      microglobulin are negative in melanomas with no extrascleral extension and liver 
      metastases and weak in melanomas with extrascleral extension and are positive in 
      melanomas with liver metastases. HLA expression is independent of the 
      conventional markers in uveal melanoma.
FAU - Krishnakumar, Subramanian
AU  - Krishnakumar S
AD  - Department of Ocular Pathology, Sankara Nethralaya, Chennai, India. 
      drkrishnakumar_2000@yahoo.com
FAU - Abhyankar, Dhiraj
AU  - Abhyankar D
FAU - Lakshmi, Sundaram Amirtha
AU  - Lakshmi SA
FAU - Pushparaj, Vaijayanthi
AU  - Pushparaj V
FAU - Shanmugam, Mahesh Palanivelu
AU  - Shanmugam MP
FAU - Biswas, Jyotirmay
AU  - Biswas J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Monoclonal
MH  - Choroid Neoplasms/*metabolism/*pathology
MH  - Eye Neoplasms/*metabolism/secondary
MH  - Female
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Liver Neoplasms/*metabolism/secondary
MH  - Lymphocytes, Tumor-Infiltrating/pathology
MH  - Male
MH  - Melanoma/*metabolism/*secondary
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Scleral Diseases/*metabolism/pathology
MH  - beta 2-Microglobulin/metabolism
EDAT- 2004/10/30 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/30 09:00
PHST- 2004/10/30 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/30 09:00 [entrez]
AID - 4A19G13B4XDJYT69 [pii]
AID - 10.1080/02713680490503741 [doi]
PST - ppublish
SO  - Curr Eye Res. 2004 Jun;28(6):409-16. doi: 10.1080/02713680490503741.