PMID- 15513363
OWN - NLM
STAT- MEDLINE
DCOM- 20041123
LR  - 20220330
IS  - 0036-5521 (Print)
IS  - 0036-5521 (Linking)
VI  - 39
IP  - 8
DP  - 2004 Aug
TI  - Prognostic relevance of 20q13 gains in sporadic colorectal cancers: a FISH 
      analysis.
PG  - 766-72
AB  - BACKGROUND: Amplification of 20q13 is a frequent chromosomal alteration in solid 
      tumors and harbors a number of putative oncogenes (CAS/CSE1-L, NABC1, or 
      Aurora2). Amplifications on 20q13 have been identified as an independent 
      prognostic marker indicating worse survival in breast and ovarian cancer. 
      However, little is known about the prognostic significance of 20q13 gains in 
      sporadic colorectal cancers. The aim of this study was to correlate 20q13 gains 
      in sporadic colorectal cancers with other known prognostic factors, tumor 
      progression, and overall survival. METHODS: Nuclei were extracted from 146 
      paraffin-embedded colorectal cancers of different UICC stages and used for 
      fluorescence in situ hybridization (FISH) with a directly labeled probe for 
      20q13.2 (VYSIS). Signals were counted in 120 nuclei per sample. 20q13 was 
      considered gained when > or =40% of the nuclei showed 3 or more FISH signals. 
      Statistical correlations were tested with log-rank tests and Kaplan-Meier 
      survival curves. RESULTS: Signal numbers for 20q13.2 were gained in 78 cases 
      (53%). Cases with gains on 20q13.2 showed worse outcome than cases without: the 
      gain of 20q13.2 was an independent prognostic marker for overall survival 
      (P=0.006) as well as tumor progression (P=0.012) in univariate and multivariate 
      analyses. Gains on 20q13.2 did not correlate with tumor stage. However, there was 
      a significant association between 20q13.2 gains and tumor location in the 
      left-sided colon and an inverse correlation between histologic grade and 20q13.2 
      gains. CONCLUSION: These data indicate that gains on 20q13.2 correlate with 
      faster tumor progression and worse patient survival independent from tumor size 
      and lymph node involvement. Therefore, alterations on 20q13 are an important 
      biological event in colorectal tumor progression with independent prognostic 
      relevance.
FAU - Aust, D E
AU  - Aust DE
AD  - Pathologisches Institut der Ludwig-Maximilians-Universitat, Munchen, Germany. 
      Daniela.Aust@pathologie.med.tu-dresden.de
FAU - Muders, M
AU  - Muders M
FAU - Kohler, A
AU  - Kohler A
FAU - Schmidt, M
AU  - Schmidt M
FAU - Diebold, J
AU  - Diebold J
FAU - Muller, C
AU  - Muller C
FAU - Lohrs, U
AU  - Lohrs U
FAU - Waldman, F M
AU  - Waldman FM
FAU - Baretton, G B
AU  - Baretton GB
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Gastroenterol
JT  - Scandinavian journal of gastroenterology
JID - 0060105
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adenoma/genetics
MH  - Chromosomes, Human, Pair 20/*genetics
MH  - Colorectal Neoplasms/chemistry/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Tumor Suppressor Protein p53/analysis
EDAT- 2004/10/30 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/30 09:00
PHST- 2004/10/30 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/30 09:00 [entrez]
AID - HJL188VYT9UACXGE [pii]
AID - 10.1080/00365520410003191 [doi]
PST - ppublish
SO  - Scand J Gastroenterol. 2004 Aug;39(8):766-72. doi: 10.1080/00365520410003191.