PMID- 15515054
OWN - NLM
STAT- MEDLINE
DCOM- 20050425
LR  - 20201209
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 70
IP  - 1
DP  - 2005 Jan
TI  - Detection and expression of hepatitis B virus X gene in one and two-cell embryos 
      from golden hamster oocytes in vitro fertilized with human spermatozoa carrying 
      HBV DNA.
PG  - 30-6
AB  - The main objectives of this study were to introduce motile human sperm carrying 
      Hepatitis B virus (HBV) DNA to golden hamster oocytes in a co-culture environment 
      and to detect the replication and expression of the HBx gene in early embryonic 
      cells. Zona-free hamster oocytes were inseminated with human sperm carrying 
      pBR322-HBV DNA plasmid using the in vitro fertilization (IVF) technique. Both the 
      one- and two-cell stages of early embryonic development were studied. PCR, 
      RT-PCR, and Dot hybridization were performed to observe the HBx gene and its 
      expression in these stages. "Fluorescence in situ hybridization" (FISH) was 
      carried out to confirm the integration of HBV into the pronucleus, nucleus, and 
      the chromosomes of embryos. The results showed that we have the ability to obtain 
      a fertilization rate of 80%. RT-PCR showed that the HBx gene could be expressed 
      in both one- and two-cell stages of embryonic development. The data suggested the 
      possibility of sperm as a vector for the vertical transmission of HBV DNA to the 
      next generation.
CI  - (c) 2004 Wiley-Liss, Inc.
FAU - Ali, Bahy-Ahmed
AU  - Ali BA
AD  - Research Center for Reproductive Medicine, Department of Cell Biology and 
      Genetics, Shantou University Medical College, Shantou, Guangdong, China.
FAU - Huang, Tian-Hua
AU  - Huang TH
FAU - Xie, Qing-Dong
AU  - Xie QD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Trans-Activators)
RN  - 0 (Viral Regulatory and Accessory Proteins)
RN  - 0 (hepatitis B virus X protein)
SB  - IM
MH  - Animals
MH  - Cricetinae
MH  - DNA, Viral/analysis/genetics
MH  - Embryo, Nonmammalian/chemistry/cytology/virology
MH  - Female
MH  - *Fertilization
MH  - Fertilization in Vitro
MH  - Gene Expression
MH  - Genes, Viral/genetics
MH  - Hepatitis B/transmission
MH  - Hepatitis B virus/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Infectious Disease Transmission, Vertical
MH  - Male
MH  - Oocytes/chemistry/metabolism
MH  - RNA, Viral/analysis/metabolism
MH  - Spermatozoa/chemistry/metabolism/*virology
MH  - Trans-Activators/*genetics/metabolism
MH  - Viral Regulatory and Accessory Proteins
EDAT- 2004/10/30 09:00
MHDA- 2005/04/26 09:00
CRDT- 2004/10/30 09:00
PHST- 2004/10/30 09:00 [pubmed]
PHST- 2005/04/26 09:00 [medline]
PHST- 2004/10/30 09:00 [entrez]
AID - 10.1002/mrd.20185 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2005 Jan;70(1):30-6. doi: 10.1002/mrd.20185.