PMID- 15515061
OWN - NLM
STAT- MEDLINE
DCOM- 20050425
LR  - 20220316
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 70
IP  - 1
DP  - 2005 Jan
TI  - Anti-apoptotic action of stem cell factor on oocytes in primordial follicles and 
      its signal transduction.
PG  - 82-90
AB  - Stem cell factor (SCF) is essential for the development of primordial follicles. 
      One of its functions is to prevent oocytes from apoptosis. However, the 
      underlying mechanism remains largely unknown. By using cultured ovaries that are 
      rich in primordial follicles, the anti-apoptotic action of SCF and the potential 
      signal transduction pathways were investigated. The apoptosis was evaluated by 
      means of in situ 3'-end labeling. The expressions of proteins were analyzed with 
      immunohistochemistry and Western blot. The data showed that SCF significantly 
      prevented oocytes from apoptosis in the cultured organs. Addition of a specific 
      pharmacological inhibitor of PI3K abolished the anti-apoptotic action of SCF 
      while that of a MEK inhibitor did not. The phosphorylation of two mitogen 
      activated protein kinases (MAPKs) (p42 and p44) and AKT, the respective 
      substrates of MEK and PI3K, were enhanced by SCF treatment. Not surprisingly, the 
      MAPK activation occurred only in theca cells. The expressions of 
      apoptosis-related gene products, the Bcl-2 family proteins, in response to SCF 
      treatment were also investigated. While SCF up-regulated the expression of the 
      anti-apoptotic proteins Bcl-2 and Bcl-xL, it did the opposite to the 
      pro-apoptotic factor Bax. The PI3K inhibitor reversed the regulation of SCF on 
      Bcl-xL and Bax but not on Bcl-2. Therefore, it seemed that SCF initiated an 
      anti-apoptotic signal starting from its membrane receptor c-kit to Bcl-2 family 
      members through PI3K/AKT and other signaling cascades in the oocytes of 
      primordial follicles.
CI  - (c) 2004 Wiley-Liss, Inc.
FAU - Jin, Xuan
AU  - Jin X
AD  - State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese 
      Academy of Sciences, Beijing, China.
FAU - Han, Chun-Sheng
AU  - Han CS
FAU - Yu, Fu-Qing
AU  - Yu FQ
FAU - Wei, Peng
AU  - Wei P
FAU - Hu, Zhao-Yuan
AU  - Hu ZY
FAU - Liu, Yi-Xun
AU  - Liu YX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Stem Cell Factor)
RN  - 0 (protein kinase modulator)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects/physiology
MH  - Female
MH  - Intracellular Signaling Peptides and Proteins/pharmacology
MH  - Oocytes/chemistry/*drug effects
MH  - Organ Culture Techniques
MH  - Ovarian Follicle/chemistry/*drug effects
MH  - Phosphatidylinositol 3-Kinases/metabolism/physiology
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/analysis/antagonists & inhibitors/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/analysis/metabolism
MH  - Rats
MH  - *Signal Transduction/drug effects/physiology
MH  - Stem Cell Factor/*pharmacology/physiology
EDAT- 2004/10/30 09:00
MHDA- 2005/04/26 09:00
CRDT- 2004/10/30 09:00
PHST- 2004/10/30 09:00 [pubmed]
PHST- 2005/04/26 09:00 [medline]
PHST- 2004/10/30 09:00 [entrez]
AID - 10.1002/mrd.20142 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2005 Jan;70(1):82-90. doi: 10.1002/mrd.20142.