PMID- 15518595
OWN - NLM
STAT- MEDLINE
DCOM- 20060731
LR  - 20181113
IS  - 1742-4690 (Electronic)
IS  - 1742-4690 (Linking)
VI  - 1
DP  - 2004 Nov 1
TI  - Alteration of T cell immunity by lentiviral transduction of human 
      monocyte-derived dendritic cells.
PG  - 37
AB  - BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that 
      play important roles during human immunodeficiency virus type 1 (HIV-1) 
      infection. HIV-1 derived lentiviral vectors (LVs) transduce DCs at high 
      efficiency but their effects on DC functions have not been carefully studied. 
      Modification of DCs using LVs may lead to important applications in 
      transplantation, treatment of cancer, autoimmune and infectious diseases. 
      RESULTS: Using DCs prepared from multiple blood donors, we report that LV 
      transduction of DCs resulted in altered DC phenotypes and functions. Lentiviral 
      transduction of DCs resulted in down-regulation of cell surface molecules 
      including CD1a, co-stimulatory molecules CD80, CD86, ICAM-1, and DC-SIGN. DCs 
      transduced with LVs displayed a diminished capacity to polarize naive T cells to 
      differentiate into Th1 effectors. This impaired Th1 response could be fully 
      corrected by co-transduction of DCs with LVs encoding interleukin-12 (IL-12), 
      interferon-gamma (IFN-gamma), or small interfering RNA (siRNA) targeting IL-10. 
      CONCLUSIONS: DCs transduced with LVs in vitro displayed diminished Th1 functions 
      due to altered DC phenotypes. Our study addresses an important issue concerning 
      lentiviral infection and modification of DC functions, and provides a rational 
      approach using LVs for immunotherapy.
FAU - Chen, Xiaochuan
AU  - Chen X
AD  - Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center, 
      McKnight Brain Institute, University of Florida College of Medicine Gainesville, 
      FL 32610-0266, USA. xchen@ufl.edu
FAU - He, Jin
AU  - He J
FAU - Chang, Lung-Ji
AU  - Chang LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20041101
PL  - England
TA  - Retrovirology
JT  - Retrovirology
JID - 101216893
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - CD4-Positive T-Lymphocytes/cytology/immunology
MH  - DNA Primers
MH  - Dendritic Cells/immunology/*virology
MH  - Flow Cytometry
MH  - Genetic Vectors
MH  - Humans
MH  - *Immunity, Cellular
MH  - Lentivirus/genetics/*immunology
MH  - Molecular Sequence Data
MH  - Monocytes/immunology/virology
MH  - T-Lymphocytes/*immunology/virology
MH  - Transduction, Genetic
PMC - PMC534092
EDAT- 2004/11/03 09:00
MHDA- 2006/08/01 09:00
PMCR- 2004/11/01
CRDT- 2004/11/03 09:00
PHST- 2004/06/28 00:00 [received]
PHST- 2004/11/01 00:00 [accepted]
PHST- 2004/11/03 09:00 [pubmed]
PHST- 2006/08/01 09:00 [medline]
PHST- 2004/11/03 09:00 [entrez]
PHST- 2004/11/01 00:00 [pmc-release]
AID - 1742-4690-1-37 [pii]
AID - 10.1186/1742-4690-1-37 [doi]
PST - epublish
SO  - Retrovirology. 2004 Nov 1;1:37. doi: 10.1186/1742-4690-1-37.