PMID- 15518646 OWN - NLM STAT- MEDLINE DCOM- 20050125 LR - 20220410 IS - 0006-8993 (Print) IS - 0006-8993 (Linking) VI - 1028 IP - 1 DP - 2004 Nov 26 TI - Altered expression of BDNF and its high-affinity receptor TrkB in response to complex motor learning and moderate exercise. PG - 92-104 AB - We report that rats learning complex motor skills or exercising moderately show changes in expression of brain-derived neurotrophic factor (BDNF) and its receptor, TrkB protein, in cerebellum and motor cortex. It is now known that physical activity increases expression of some neurotrophins. We examined the time course of BDNF and TrkB expression after 1, 3, 5, 7 or 14 days in one of three conditions: (1) an "acrobatic" motor skill learning condition (AC), (2) a motor activity condition (moderately paced running on a flat track; MC) and (3) an inactive social-only control (SC) that served as a baseline group. Expression levels of BDNF and TrkB were evaluated by measuring relative optical density of the immunocytochemical reaction product. In cerebellar molecular layer, expression of BDNF correlated significantly with time spent in AC and MC over the first 7 days of training and remained elevated after 14 days of AC but not of MC. Changes in TrkB protein expression in cerebellar molecular layer mirrored those for BDNF during the first 7 days of training, but subsequently its expression subsided to the control level. In motor cortex, a significant increase in BDNF and TrkB protein expression was detected in the upper layers after 14 days in AC. Increased expression of BDNF, but not of TrkB, was observed in upper motor cortical layers after 14 days of MC. These data indicate that complex motor learning and moderate physical activity with little learning produce different effects on the expression pattern of BDNF and its receptor and may have implications for neural plasticity arising from such experiences. FAU - Klintsova, Anna Y AU - Klintsova AY AD - Department of Psychology, Binghamton University, P.O. Box 6000, NY 13902, USA. aklintso@binghamton.edu FAU - Dickson, Elizabeth AU - Dickson E FAU - Yoshida, Rie AU - Yoshida R FAU - Greenough, William T AU - Greenough WT LA - eng GR - R01 AA009838/AA/NIAAA NIH HHS/United States GR - AA09838/AA/NIAAA NIH HHS/United States GR - AG10154/AG/NIA NIH HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (Brain-Derived Neurotrophic Factor) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Analysis of Variance MH - Animals MH - Association Learning/physiology MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cerebellum/*metabolism MH - Dentate Gyrus/metabolism MH - Female MH - Motor Activity MH - Motor Cortex/*metabolism MH - Motor Skills/*physiology MH - Neuronal Plasticity/physiology MH - Physical Conditioning, Animal/*physiology MH - Rats MH - Rats, Long-Evans MH - Receptor, trkB/*metabolism MH - Social Behavior MH - Time Factors EDAT- 2004/11/03 09:00 MHDA- 2005/01/26 09:00 CRDT- 2004/11/03 09:00 PHST- 2004/09/06 00:00 [accepted] PHST- 2004/11/03 09:00 [pubmed] PHST- 2005/01/26 09:00 [medline] PHST- 2004/11/03 09:00 [entrez] AID - S0006-8993(04)01500-8 [pii] AID - 10.1016/j.brainres.2004.09.003 [doi] PST - ppublish SO - Brain Res. 2004 Nov 26;1028(1):92-104. doi: 10.1016/j.brainres.2004.09.003.