PMID- 15520023
OWN - NLM
STAT- MEDLINE
DCOM- 20050531
LR  - 20061115
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 19
IP  - 12
DP  - 2004 Dec
TI  - Aneuploidy study of human oocytes first polar body comparative genomic 
      hybridization and metaphase II fluorescence in situ hybridization analysis.
PG  - 2859-68
AB  - BACKGROUND: The object of this study was to determine the mechanisms that produce 
      aneuploidy in oocytes and establish which chromosomes are more prone to 
      aneuploidy. METHODS: A total of 54 oocytes from 36 women were analysed. The whole 
      chromosome complement of the first polar body (1PB) was analysed by comparative 
      genomic hybridization (CGH), while the corresponding metaphase II (MII) oocyte 
      was analysed by fluorescence in situ hybridization (FISH) to confirm the results. 
      RESULTS: Matched CGH-FISH results were obtained in 42 1PB-MII doublets, of which 
      37 (88.1%) showed reciprocal results. The aneuploidy rate was 57.1%. Two-thirds 
      of the aneuploidy events were chromatid abnormalities. Interestingly, the 
      chromosomes more frequently involved in aneuploidy were chromosomes 1, 4 and 22 
      followed by chromosome 16. In general, small chromosomes (those equal to or 
      smaller in size than chromosome 13) were more prone to aneuploidy (chi2-test, 
      P=0.07); 25% of the aneuploid doublets would have been misdiagnosed as normal 
      using FISH with probes for nine-chromosomes. CONCLUSIONS: The combination of two 
      different techniques, CGH and FISH, for the study of 1PB and MII allowed the 
      identification and confirmation of any numerical chromosome abnormality, as well 
      as helping to determine the mechanisms involved in the genesis of maternal 
      aneuploidy.
FAU - Gutierrez-Mateo, C
AU  - Gutierrez-Mateo C
AD  - Departament de Biologia Cel.lular, Fisiologia i Immunologia, Unitat de Medicina, 
      Universitat Autonoma de Barcelona, E-08193 Bellaterra, Spain. 
      cristina.gutierrez@uab.es
FAU - Benet, J
AU  - Benet J
FAU - Wells, D
AU  - Wells D
FAU - Colls, P
AU  - Colls P
FAU - Bermudez, M G
AU  - Bermudez MG
FAU - Sanchez-Garcia, J F
AU  - Sanchez-Garcia JF
FAU - Egozcue, J
AU  - Egozcue J
FAU - Navarro, J
AU  - Navarro J
FAU - Munne, S
AU  - Munne S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041101
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - *Aneuploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase/*genetics
MH  - Oocytes/*physiology
EDAT- 2004/11/03 09:00
MHDA- 2005/06/01 09:00
CRDT- 2004/11/03 09:00
PHST- 2004/11/03 09:00 [pubmed]
PHST- 2005/06/01 09:00 [medline]
PHST- 2004/11/03 09:00 [entrez]
AID - deh515 [pii]
AID - 10.1093/humrep/deh515 [doi]
PST - ppublish
SO  - Hum Reprod. 2004 Dec;19(12):2859-68. doi: 10.1093/humrep/deh515. Epub 2004 Nov 1.