PMID- 15522599
OWN - NLM
STAT- MEDLINE
DCOM- 20050511
LR  - 20191210
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 20
IP  - 4
DP  - 2004 Nov 1
TI  - Development of agmatine sensor using the combination of putrescine oxidase and 
      agmatinase for squid freshness.
PG  - 833-40
AB  - Agmatine (Agm) is an indicator of squid freshness. The Agm sensor was developed 
      using flow injection analysis (FIA) that consisted of the putrescine oxidase 
      (PuOx) reactor, the agmatinase (AUH)-PuOx reactor and two oxygen electrodes. In 
      the proposed sensor, the first step is that coexisting cadaverine (Cad) and 
      putrescine (Put) are removed by passing through the PuOx reactor and the initial 
      decomposition is determined by the amount of oxygen consumed, simultaneously. The 
      second step is that the amount of Agm is determined by the amount of oxygen 
      consumed in the AUH-PuOx reactor. The optimum conditions for the use of the Agm 
      sensor were as follows: 50 mM HEPES containing MnSO4 at a final concentration of 
      5 mM, pH 8.0, flow rate of 0.6 mL min(-1) and injection volume of 50 microL. A 
      single assay could be completed in approximately 3 min. A linear relationship was 
      obtained between the output and the Agm concentration in the range of 0.01-1 mM 
      Agm with a correlation coefficient of 0.999. The detection limit was 0.005 mM. 
      The relative standard deviations (RSDs) were 3.14 and 1.19% (n = 20) for 0.1 and 
      0.3 mM Agm, respectively. The extracts of squid were injected into the proposed 
      sensor and the results were compared with those obtained using the conventional 
      high-performance liquid chromatography (HPLC) method. A correlation was observed 
      between the results obtained by the proposed sensor and those obtained by the 
      conventional method. The determination of squid freshness is one of the good uses 
      of the proposed Agm sensor.
FAU - Inaba, Yohei
AU  - Inaba Y
AD  - Department of Food Science and Technology, Tokyo University of Fisheries, 5-7 
      Kounan 4, Minato-ku, Tokyo 108-8477, Japan.
FAU - Tokishita, Shinichi
AU  - Tokishita S
FAU - Hamada-Sato, Naoko
AU  - Hamada-Sato N
FAU - Kobayashi, Takeshi
AU  - Kobayashi T
FAU - Imada, Chiaki
AU  - Imada C
FAU - Yamagata, Hideo
AU  - Yamagata H
FAU - Watanabe, Etsuo
AU  - Watanabe E
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 70J407ZL5Q (Agmatine)
RN  - EC 1.4.3.10 (putrescine oxidase)
RN  - EC 1.5.- (Oxidoreductases Acting on CH-NH Group Donors)
RN  - EC 3.5.3.- (Ureohydrolases)
RN  - EC 3.5.3.11 (agmatinase)
SB  - IM
MH  - Agmatine/*analysis
MH  - Animals
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Decapodiformes/*chemistry
MH  - Electrochemistry/*instrumentation/methods
MH  - Flow Injection Analysis/instrumentation/methods
MH  - Food Analysis/*instrumentation/methods
MH  - Microchemistry/instrumentation/methods
MH  - Oxidoreductases Acting on CH-NH Group Donors/*chemistry
MH  - Systems Integration
MH  - Ureohydrolases/*chemistry
EDAT- 2004/11/04 09:00
MHDA- 2005/05/12 09:00
CRDT- 2004/11/04 09:00
PHST- 2003/09/30 00:00 [received]
PHST- 2004/02/28 00:00 [revised]
PHST- 2004/03/24 00:00 [accepted]
PHST- 2004/11/04 09:00 [pubmed]
PHST- 2005/05/12 09:00 [medline]
PHST- 2004/11/04 09:00 [entrez]
AID - S0956566304001538 [pii]
AID - 10.1016/j.bios.2004.03.022 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2004 Nov 1;20(4):833-40. doi: 10.1016/j.bios.2004.03.022.