PMID- 15522704
OWN - NLM
STAT- MEDLINE
DCOM- 20050210
LR  - 20161124
IS  - 1569-9048 (Print)
IS  - 1569-9048 (Linking)
VI  - 144
IP  - 1
DP  - 2004 Nov 30
TI  - HIF and VEGF relationships in response to hypoxia and sciatic nerve stimulation 
      in rat gastrocnemius.
PG  - 71-80
AB  - To determine if hypoxia-inducible factor-1 (HIF-1) may regulate skeletal muscle 
      vascular endothelial growth factor (VEGF) expression in response to exercise or 
      hypoxia, rats underwent 1h sciatic nerve electrical stimulation (ES), hypoxic 
      exposure (H) or combined stimuli. HIF-1alpha protein levels increased six-fold 
      with maximal (8V) ES with or without H. Similar HIF-1alpha increases occurred 
      with sub-maximal (6V and 4V) ES plus H, but not in sub-maximal ES or H alone. 
      VEGF mRNA and protein levels increased three-fold in sub-maximal ES or H alone, 
      six-fold in sub-maximal ES plus H, 6.3-fold with maximal ES, and 6.5-fold after 
      maximal ES plus H. These data suggest: (1) intracellular hypoxia during normoxic 
      exercise may exceed that during 8% oxygen breathing at rest and is more effective 
      in stimulating HIF-1alpha; (2) HIF-1 may be an important regulator of 
      exercise-induced VEGF transcription; and (3) breathing 8% O(2) does not alter 
      HIF-1alpha expression in skeletal muscle, implying that exercise-generated 
      signals contribute to the regulation of HIF-1alpha and/or VEGF.
FAU - Tang, Kechun
AU  - Tang K
AD  - Division of Physiology (0623A), Department of Medicine, University of California 
      at San Diego, La Jolla, CA 92093, USA. ktang@ucsd.edu
FAU - Breen, Ellen C
AU  - Breen EC
FAU - Wagner, Harrieth
AU  - Wagner H
FAU - Brutsaert, Tom D
AU  - Brutsaert TD
FAU - Gassmann, Max
AU  - Gassmann M
FAU - Wagner, Peter D
AU  - Wagner PD
LA  - eng
GR  - HL17731/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Respir Physiol Neurobiol
JT  - Respiratory physiology & neurobiology
JID - 101140022
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Electric Stimulation
MH  - Female
MH  - Gene Expression Regulation
MH  - Hypoxia/*metabolism
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Muscle Contraction/physiology
MH  - Muscle, Skeletal/innervation/*metabolism
MH  - Nuclear Proteins/genetics/*metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Reference Values
MH  - Sciatic Nerve/*physiology
MH  - Transcription Factors/genetics/*metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
EDAT- 2004/11/04 09:00
MHDA- 2005/02/11 09:00
CRDT- 2004/11/04 09:00
PHST- 2004/04/14 00:00 [accepted]
PHST- 2004/11/04 09:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/11/04 09:00 [entrez]
AID - S1569904804000862 [pii]
AID - 10.1016/j.resp.2004.04.009 [doi]
PST - ppublish
SO  - Respir Physiol Neurobiol. 2004 Nov 30;144(1):71-80. doi: 
      10.1016/j.resp.2004.04.009.