PMID- 15522725
OWN - NLM
STAT- MEDLINE
DCOM- 20050324
LR  - 20151119
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 811
IP  - 2
DP  - 2004 Nov 25
TI  - Development of a Micellar electrokinetic capillary chromatography method for the 
      determination of three drugs employed in the erectile dysfunction therapy.
PG  - 231-6
AB  - A Micellar electrokinetic capillary chromatography method is proposed for the 
      determination of sildenafil, vardenafil and tadalafil, which are employed in oral 
      therapy for erectile dysfunction. Optimum conditions for the separation were 
      investigated. A background electrolyte solution consisting of 10 mM phosphate 
      buffer adjusted to pH 12.0, sodium dodecyl sulfate (SDS) 25 mM, hydrodynamic 
      injection, and 25 kV as separation voltage were used. Relative standard 
      deviations (R.S.D.s) were 1.0, 1.0, 0.4% and 2.9, 2.9, 1.9% for migration time 
      and corrected peak area (CPA) (n = 9) for sildenafil, vardenafil and tadalafil, 
      respectively. Detection limits obtained for the three drugs ranged from 0.19 to 
      0.61 mg L(-1). A linear concentration range between 1 and 20 mg L(-1) was 
      obtained. A ruggedness test of this method was checked using the fractional 
      factorial model of Plackett-Burman, in which the influence of six factors at 
      three different levels was tested on different electrophoretic results: 
      resolution and corrected peak area. The statistical evaluation of the 
      electrophoretic results was achieved by Youden and Steiner method. The described 
      method is rapid, sensitive and rugged and it was tested in the pharmaceutical 
      formulations analysis obtaining recoveries between 98 and 107% respect to the 
      nominal content.
FAU - Rodriguez Flores, J
AU  - Rodriguez Flores J
AD  - Department of Analytical Chemistry and Foods Technology, UCLM 13071, Ciudad Real, 
      Spain. juana.rflores@uclm.es
FAU - Berzas Nevado, J J
AU  - Berzas Nevado JJ
FAU - Castaneda Penalvo, G
AU  - Castaneda Penalvo G
FAU - Mora Diez, N
AU  - Mora Diez N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Carbolines)
RN  - 0 (Imidazoles)
RN  - 0 (Piperazines)
RN  - 0 (Purines)
RN  - 0 (Sulfones)
RN  - 0 (Triazines)
RN  - 5O8R96XMH7 (Vardenafil Dihydrochloride)
RN  - 742SXX0ICT (Tadalafil)
RN  - BW9B0ZE037 (Sildenafil Citrate)
SB  - IM
MH  - Carbolines/*analysis
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Erectile Dysfunction/*drug therapy
MH  - Humans
MH  - Imidazoles/*analysis
MH  - Male
MH  - Piperazines/*analysis
MH  - Purines
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Sildenafil Citrate
MH  - Sulfones/*analysis
MH  - Tadalafil
MH  - Triazines/*analysis
MH  - Vardenafil Dihydrochloride
EDAT- 2004/11/04 09:00
MHDA- 2005/03/25 09:00
CRDT- 2004/11/04 09:00
PHST- 2004/04/02 00:00 [received]
PHST- 2004/07/13 00:00 [accepted]
PHST- 2004/11/04 09:00 [pubmed]
PHST- 2005/03/25 09:00 [medline]
PHST- 2004/11/04 09:00 [entrez]
AID - S1570-0232(04)00589-6 [pii]
AID - 10.1016/j.jchromb.2004.07.016 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 25;811(2):231-6. doi: 
      10.1016/j.jchromb.2004.07.016.