PMID- 15525462
OWN - NLM
STAT- MEDLINE
DCOM- 20050318
LR  - 20181201
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 25
IP  - 11
DP  - 2004 Nov
TI  - Homocysteine induces production of monocyte chemoattractant protein-1 and 
      interleukin-8 in cultured human whole blood.
PG  - 1419-25
AB  - AIM: To investigate whether increased plasma L-homocysteine (Hcy) level could 
      promote monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in 
      cultured whole blood. METHODS: Human whole blood or different type of peripheral 
      blood cells from health volunteers were incubated with Hcy and/or the inhibitors. 
      MCP-1 and IL-8 level were measured by ELISA assay. RESULTS: Hcy 10-1000 
      micromol/L induced production of MCP-1 and IL-8 in cultured human whole blood 
      (P<0.05). The major cellular source of these chemokines comed from monocytes. 
      Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 
      micromol/L in the cultured whole blood. The intracellular ROS, particular the OH. 
      radicals, play extremely important role in the Hcy-induced MCP-1 and IL-8 
      production. CONCLUSION: Increased Hcy level in plasma (hyperhomocysteinemia) 
      induced MCP-1 and IL-8 secretion in cultured human whole blood, especially in 
      monocytes via oxidative stress mechanism.
FAU - Zeng, Xiao-kun
AU  - Zeng XK
AD  - Institute of Vascular Medicine, Peking University Third Hospital, China.
FAU - Remick, Daniel G
AU  - Remick DG
FAU - Wang, Xian
AU  - Wang X
LA  - eng
GR  - GM50401/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Reactive Oxygen Species)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Homocysteine/*pharmacology
MH  - Humans
MH  - Interleukin-8/*metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Monocytes/*metabolism
MH  - Peroxidase/metabolism
MH  - Reactive Oxygen Species/metabolism
EDAT- 2004/11/05 09:00
MHDA- 2005/03/19 09:00
CRDT- 2004/11/05 09:00
PHST- 2004/11/05 09:00 [pubmed]
PHST- 2005/03/19 09:00 [medline]
PHST- 2004/11/05 09:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2004 Nov;25(11):1419-25.